Heparan sulfate facilitates Rift Valley fever virus entry into the cell.
Authorsde Boer SM, Kortekaas J, de Haan CA, Rottier PJ, Moormann RJ, Bosch BJ,
JournalJ Virol
PubMed ID23015725
Rift Valley fever virus (RVFV), an emerging arthropod-borne pathogen, has a broad host and cell tropism. Here we report that the glycosaminoglycan heparan sulfate, abundantly present on the surface of most animal cells, is required for efficient entry of RVFV. Entry was significantly reduced by preincubating the virus inoculum with ... More
Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of glaucoma.
AuthorsStankowska DL, Minton AZ, Rutledge MD, Mueller BH, Phatak NR, He S, Ma HY, Forster MJ, Yorio T, Krishnamoorthy RR,
Journal
PubMed ID25587060
'Purpose: Glaucoma is an optic neuropathy commonly associated with elevated intraocular pressure (IOP), leading to optic nerve head (ONH) cupping, axon loss and apoptosis of retinal ganglion cells (RGCs) which could ultimately result in blindness. Brn3b is a class-4 POU domain transcription factor that plays a key role in RGC ... More
Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity.
AuthorsKrajewska M, You Z, Rong J, Kress C, Huang X, Yang J, Kyoda T, Leyva R, Banares S, Hu Y, Sze CH, Whalen MJ, Salmena L, Hakem R, Head BP, Reed JC, Krajewski S,
JournalPLoS One
PubMed ID21957448
'Acute brain injury is an important health problem. Given the critical position of caspase 8 at the crossroads of cell death pathways, we generated a new viable mouse line (Ncasp8(-/-)), in which the gene encoding caspase 8 was selectively deleted in neurons by cre-lox system. Caspase 8 deletion reduced rates ... More
PARK9-associated ATP13A2 localizes to intracellular acidic vesicles and regulates cation homeostasis and neuronal integrity.
AuthorsRamonet D, Podhajska A, Stafa K, Sonnay S, Trancikova A, Tsika E, Pletnikova O, Troncoso JC, Glauser L, Moore DJ,
JournalHum Mol Genet
PubMed ID22186024
'Mutations in the ATP13A2 gene (PARK9, OMIM 610513) cause autosomal recessive, juvenile-onset Kufor-Rakeb syndrome and early-onset parkinsonism. ATP13A2 is an uncharacterized protein belonging to the P(5)-type ATPase subfamily that is predicted to regulate the membrane transport of cations. The physiological function of ATP13A2 in the mammalian brain is poorly understood. ... More
Cofilin nuclear-cytoplasmic shuttling affects cofilin-actin rod formation during stress.
AuthorsMunsie LN, Desmond CR, Truant R,
JournalJ Cell Sci
PubMed ID22623727
'Cofilin protein is involved in regulating the actin cytoskeleton during typical steady state conditions, as well as during cell stress conditions where cofilin saturates F-actin, forming cofilin-actin rods. Cofilin can enter the nucleus through an active nuclear localization signal (NLS), accumulating in nuclear actin rods during stress. Here, we characterize ... More
The Vpx lentiviral accessory protein targets SAMHD1 for degradation in the nucleus.
AuthorsHofmann H, Logue EC, Bloch N, Daddacha W, Polsky SB, Schultz ML, Kim B, Landau NR,
JournalJ Virol
PubMed ID22973040
'Sterile alpha motif domain- and HD domain-containing protein 1 (SAMHD1) is a deoxynucleoside triphosphohydrolase that restricts the replication of lentiviruses in myeloid cells by hydrolyzing the cellular deoxynucleotide triphosphates to a level below that which is required for reverse transcription. Human immunodeficiency virus type 2 (HIV-2) and some simian immunodeficiency ... More
CD47 functions as a molecular switch for erythrocyte phagocytosis.
AuthorsBurger P, Hilarius-Stokman P, de Korte D, van den Berg TK, van Bruggen R,
JournalBlood
PubMed ID22427202
'CD47 on erythrocytes inhibits phagocytosis through interaction with the inhibitory immunoreceptor SIRPa expressed by macrophages. Thus, the CD47-SIRPa interaction constitutes a negative signal for erythrocyte phagocytosis. However, we report here that CD47 does not only function as a ' ... More
Vascular endothelial growth factor A (VEGF-A) regulates many aspects of vascular physiology. VEGF-A stimulates signal transduction pathways that modulate endothelial outputs such as cell migration, proliferation, tubulogenesis and cell-cell interactions. Multiple VEGF-A isoforms exist but the biological significance is unclear. Here we analyzed VEGF-A isoform-specific stimulation of VCAM-1 gene expression ... More
Mammalian target of rapamycin complex 1 (mTORC1) plays a role in Pasteurella multocida toxin (PMT)-induced protein synthesis and proliferation in Swiss 3T3 cells.
AuthorsOubrahim H, Wong A, Wilson BA, Chock PB,
JournalJ Biol Chem
PubMed ID23223576
Pasteurella multocida toxin (PMT) is a potent mitogen known to activate several signaling pathways via deamidation of a conserved glutamine residue in the a subunit of heterotrimeric G-proteins. However, the detailed mechanism behind mitogenic properties of PMT is unknown. Herein, we show that PMT induces protein synthesis, cell migration, and ... More
Proliferation of genetically modified human cells on electrospun nanofiber scaffolds.
Gene editing is a process by which single base mutations can be corrected, in the context of the chromosome, using single-stranded oligodeoxynucleotides (ssODNs). The survival and proliferation of the corrected cells bearing modified genes, however, are impeded by a phenomenon known as reduced proliferation phenotype (RPP); this is a barrier ... More
The
AuthorsHorst D, Burmeister WP, Boer IG, van Leeuwen D, Buisson M, Gorbalenya AE, Wiertz EJ, Ressing ME,
JournalJ Virol
PubMed ID22696660
Replication of the human herpesvirus Epstein-Barr virus drastically impairs cellular protein synthesis. This shutoff phenotype results from mRNA degradation upon expression of the early lytic-phase protein BGLF5. Interestingly, BGLF5 is the viral DNase, or alkaline exonuclease, homologues of which are present throughout the herpesvirus family. During productive infection, this DNase ... More
Heat shock and caloric restriction have a synergistic effect on the heat shock response in a sir2.1-dependent manner in Caenorhabditis elegans.
The heat shock response (HSR) is responsible for maintaining cellular and organismal health through the regulation of proteostasis. Recent data demonstrating that the mammalian HSR is regulated by SIRT1 suggest that this response may be under metabolic control. To test this hypothesis, we have determined the effect of caloric restriction ... More
Tissue factor–dependent procoagulant activity of subtilase cytotoxin, a potent AB5 toxin produced by shiga toxigenic Escherichia coli.
AuthorsWang H, Paton JC, Thorpe CM, Bonder CS, Sun WY, Paton AW,
JournalJ Infect Dis
PubMed ID20874089
Subtilase cytotoxin (SubAB), produced by certain virulent Shiga toxigenic Escherichia coli strains, causes hemolytic uremic syndrome-like pathology in mice, including extensive microvascular thrombosis. SubAB acts by specifically cleaving the essential endoplasmic reticulum chaperone binding immunoglobulin protein (BiP). BiP has been reported to inhibit the activation of tissue factor (TF), the ... More
Resolvin D1 decreases adipose tissue macrophage accumulation and improves insulin sensitivity in obese-diabetic mice.
AuthorsHellmann J, Tang Y, Kosuri M, Bhatnagar A, Spite M,
JournalFASEB J
PubMed ID21478260
Type 2 diabetes and obesity have emerged as global public health crises. Adipose tissue expansion in obesity promotes accumulation of classically activated macrophages that perpetuate chronic inflammation and sustain insulin resistance. Acute inflammation normally resolves in an actively orchestrated series of molecular and cellular events that ensures return to homeostasis ... More
Gap junction-mediated import of microRNA from bone marrow stromal cells can elicit cell cycle quiescence in breast cancer cells.
AuthorsLim PK, Bliss SA, Patel SA, Taborga M, Dave MA, Gregory LA, Greco SJ, Bryan M, Patel PS, Rameshwar P,
JournalCancer Res
PubMed ID21343399
Bone marrow (BM) metastasis of breast cancer (BC) can recur even decades after initial diagnosis and treatment, implying the long-term survival of disseminated cancer cells in a dormant state. Here we investigated the role of microRNAs (miRNA) transmitted from BM stroma to BC cells via gap junctions and exosomes in ... More