Thermo Scientific™

Lab Vision™ Protease XXV for Enzyme-Induced Epitope Retrieval

 Related applications:
Anatomical Pathology
Improve the immunoreactivity of certain antigens in formaldehyde-fixed tissues with Thermo Scientific™ Lab Vision™ Protease XXV for Enzyme-Induced Epitope Retrieval.

Error loading your content!

  Catalog number Price ({{currency}}) Availability Qty
{{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}} Add To Cart

{{addItemToList.successMessage}} {{addItemToList.listName}}


Back to top

Description

Storage Conditions: Store at 2°-8°C. Stable for 18 months

For in vitro diagnostic use
Procedure:

1. Place five-micron thick tissue sections on glass slides.

2. Deparaffinize and rehydrate sections as usual.

3. Cover sections with Protease XXV solution and digest for 10 minutes at 37°C.

4. Wash sections in PBS for 2 x 5 minutes.

5. Block endogenous peroxidase as usual.

6. Wash sections in PBS for 2 x 5 minutes.

7. Block nonspecific sites with normal serum as usual.

8. Place optimally diluted primary antibody on the sections (incubation time and temperature for a given set of experimental conditions should be determined by the investigator).

9. Wash sections in PBS for 2 x 5 minutes.

10. Remaining procedure is same as routinely performed in your laboratory.



Documents

Manuals & protocols