Luminex™ 200™ Instrument System - FAQs

View additional product information for Luminex™ 200™ Instrument System - FAQs (APX10031)

9 product FAQs found

How do the Luminex 200 Instrument System, Luminex FLEXMAP3D Instrument System and Luminex xMAP INTELLIFLEX System simultaneously monitor concentrations of several different analytes in a single sample?

Each protein detected requires a different bead region to differentiate between analytes. The Luminex 200 Instrument System, Luminex FLEXMAP3D Instrument System and Luminex xMAP INTELLIFLEX System are outfitted with two lasers. The first laser is a red diode laser that classifies beads (region) based on color and hence identifies which protein is being measured. The second laser is a green YAG laser that quantitates reporter fluorophore (R-Phycoerythin, RPE) associated with the bead surface and determines the amount of the protein that is bound to the bead. The beads pass through the system at a rate of several thousand per second, excited first by the red laser and then the green laser. The results obtained show protein identity and the intensity of the signal, respectively. The intensity of the signal is directly proportional to the concentration of the analyte captured in the sample. Therefore, by monitoring the spectral properties of the beads and the amount of associated RPE fluorescence, the concentration of one or more proteins can be determined.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

When using the VEGF-A Human ProcartaPlex Simplex Kit (Cat. No. EPX01A-10277-901) with the Luminex 200 Instrument System (Cat. No. APX10031), what settings should be used for the Bead Region?

We recommend using a Bead Region setting of 78.

What species can ProcartaPlex Platinum Assay Buffer for Plasma Samples be used with?

ProcartaPlex Platinum Assay Buffer for Plasma Samples is designed to be used with the ProcartaPlex Platinum assay format. Currently, this format has been validated for human samples only. Other species have not been tested.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What kits can ProcartaPlex Platinum Assay Buffer for Plasma Samples be used with?

ProcartaPlex Platinum Assay Buffer for Plasma Samples is designed to be used with the ProcartaPlex Platinum assay format. It is used for dilution of plasma samples and gives spike- and dilution-recovery results in the range of 70-130%. ProcartaPlex Platinum Assay Buffer for Plasma Samples is included in our Platinum ProcartaPlex Human Basic Kit for Plasma Samples (Cat.No. EPXP010-10421-901) and off-the-shelf Platinum panels. It is also offered as a stand-alone item (Cat. No. EPXP-11112-000).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the difference between xMAP Sheath Concentrate PLUS, RUO and xMAP Sheath Fluid Plus?

xMAP Sheath Concentrate PLUS, RUO (Cat. No. 4050023) is a 20X concentrated version of xMAP Sheath Fluid Plus (Cat. No. 4050021). It needs to be diluted before use.

xMAP Sheath Concentrate PLUS, RUO (Cat. No. 4050023) is a reformulation of xMAP Sheath Fluid Concentrate (Cat. No. 4050018). The formulation is updated from a single preservative to a mixture of three preservatives to allow for greater transit stability and to reduce the likelihood of future acquired microbial resistance.

Will there be any differences in performance of my instrument(s) when switching over to xMAP Sheath Fluid Plus from the original xMAP Sheath Fluid?

In-house testing has been done to confirm that assays deliver expected results when using xMAP Sheath Fluid Plus. White Paper is available upon request from Technical Support (techsupport@thermofisher.com).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are there special instructions or considerations when switching over to xMAP Sheath Fluid Plus from the original xMAP Sheath Fluid?

Yes, there are instructions available for flushing the instrument and replacing the sheath fluid. Please contact Technical Support at techsupport@thermofisher.com for these instructions.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the advantage of xMAP Sheath Fluid Plus over the original xMAP Sheath Fluid?

xMAP Sheath Fluid Plus (Cat. No. 4050021) replaces the original xMAP Sheath Fluid (Cat. No. 4050015). The new sheath formulation is updated from a single preservative to a mixture of three preservatives, specifically, sodium azide, sodium benzoate and diazolidinyl urea. This allows for greater transit stability and reduces the likelihood of future acquired microbial resistance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Which Luminex instruments are available for multiplex detection and analysis?

Here (https://www.thermofisher.com/us/en/home/life-science/antibodies/immunoassays/procartaplex-assays-luminex/luminex-instruments.html) is an overview of Luminex instruments we offer for multiplex detection and analysis.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.