Bolt® Western Pack A (Nitrocellulose)

Catalog number: B1000A

Novex™  Related applications: Protein Assays and Analysis | Protein Gel Electrophoresis

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Description

The Bolt® Western Pack (Nitrocellulose) includes two Mini Blot Modules and all of the buffers and pre-cut membrane/filter paper sandwiches you will need for the wet transfer of proteins from Bolt® Bis-Tris Plus gels to nitrocellulose membranes. Each pack contains:
Mini Blot Modules
Bolt® Transfer Buffer (20X)
Bolt® Antioxidant
Nitrocellulose pre-cut membranes (0.2 µm)

About the Mini Blot Module
The Mini Blot Module is a wet transfer device used exclusively with the Mini Gel Tank. The tank accommodates one blot module per chamber, or two blot modules total with the side-by-side layout. The Mini Blot Module is compatible with Bolt® Bis-Tris Plus gels and accommodates one gel per wet transfer. The universal connection and molded gasket make the blot module easy to use, while the inner core of the blot module allows for use of less methanol-based transfer buffer per wet transfer. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes typically in 30 to 60 minutes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Mode of Transfer: Wet
Gel Compatibility: Bolt® Bis-Tris Plus Gels
Running Dimension: Vertical
For Use With (Equipment): Bolt®
Capacity: ≤ 2 Blot Modules ⁄ Mini-Gel Tank, 1 Mini-Gel ⁄ Blot Module
Gel Size: Mini (8 cm x 8 cm)
Product Size: 1 kit

Contents & storage

2 Mini Blot Modules
125 mL Bolt® Transfer Buffer (20X)
15 mL Bolt® Antioxidant
20-pack nitrocellulose membranes (0.2 µm)

Documents

Manuals & protocols