Invitrogen™
BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)
Catalog number: B1150
Related applications:Description
With a pKa of ~6.98, the cell-permeant, dual-excitation ratiometric pH indicator BCECF, AM is ideal for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| For Use With (Equipment): | Microplate Reader |
|---|---|
| Quantity: | 1mg |
| Cell Permeability: | Cell-Permeant |
| Detection Method: | Fluorescent |
| Indicator Properties: | Fluorescent Dye-Based |
| Indicator: | pH Indicator |
| Shipping Condition: | Room Temperature |
Contents & storage
Store in freezer (-5 to -30°C).
