With a pKa of ∼6.98, the cell-permeant, dual-excitation ratiometric pH indicator BCECF, AM is ideal for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ∼490 nm versus the emission intensity when excited at its isobestic point of ∼440 nm.
Specifications
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Product Type
BCECF AM pH Indicator
Dye Type
pH Indicator
Quantity
1mg
Shipping Condition
Room Temperature
Contents & Storage
Store in freezer -5°C to -30°C.
Figures
Loading and retention characteristics of intracellular marker dyes.