Will BSA generate background during Lumio tag labeling for in-gel detection?
Serum proteins (BSA, 66 kDa) from the mammalian cell culture medium may cross-react with the Lumio Reagent producing non-specific bands. Removing the cell culture medium and washing the mammalian cells 3-4 times with PBS after harvesting the cells minimizes the non-specific binding from BSA. The BSA band may show up as a 66 kDa band.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.