Bovine Serum Albumin (BSA) - FAQs

Will BSA generate background during Lumio tag labeling for in-gel detection?

Serum proteins (BSA, 66 kDa) from the mammalian cell culture medium may cross-react with the Lumio Reagent producing non-specific bands. Removing the cell culture medium and washing the mammalian cells 3-4 times with PBS after harvesting the cells minimizes the non-specific binding from BSA. The BSA band may show up as a 66 kDa band.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.