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Citas y referencias (233)
Invitrogen™
Bis-ANS (4,4'-dianilino-1,1'-binaftilo-5,5'-ácido disulfónico, sal de dipotasio)
Bis-ANS es superior a 1-8-ANS como una sonda para cavidades no polares en proteínas que a menudo se une conMás información
| Número de catálogo | Cantidad |
|---|---|
| B153 | 10 mg |
Número de catálogo B153
Precio (MXN)
-
Cantidad:
10 mg
Bis-ANS es superior a 1-8-ANS como una sonda para cavidades no polares en proteínas que a menudo se une con una afinidad que es de orden de magnitud superior. Los sulfonatos anilinonaftalenos (ANS) esencialmente no fluorescente en el agua, solo se vuelve apreciablemente fluorescente cuando se une a las membranas Un indicador sensible de plegamiento de proteínas, cambios conformacionales y otros procesos que modifican la exposición de la sonda al agua
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de etiquetaOtras etiquetas o colorantes
Fórmula molecularC32H22K2N2O6S2
Línea de productosMolecular Probes
Cantidad10 mg
Almacenamiento recomendadoAlmacenar a temperatura ambiente y proteger de la luz.
Condiciones de envíoTemperatura ambiente
Localización subcelularMembranas celulares y lípidos
Forma físicaSólido
Tipo de productoBis-ANS
Unit SizeEach
Citations & References (233)
Citations & References
Abstract
Interaction of 1,1'-bi(4-anilino)naphthalene-5,5'-disulfonic acid with alpha-crystallin.
Journal:J Biol Chem
PubMed ID:9535881
'The hydrophobic sites in alpha-crystallin were evaluated using a fluorescent probe 1,1''-bi(4-anilino)naphthalenesulfonic acid (bis-ANS). Approximately one binding site/subunit of alpha-crystallin at 25 degrees C was estimated by equilibrium binding and Scatchard analysis (Kd = 1.1 microM). Based on fluorescence titration, the dissociation constant was 0.95 microM. The number of bis-ANS
Plurality of protein conformations of ribulose-1,5-bisphosphate carboxylase/oxygenase monomers probed by high pressure electrophoresis.
Journal:J Biol Chem
PubMed ID:7902356
'We used hydrostatic pressure in the range of 1 to 2 kbar, coupled with polyacrylamide gel electrophoresis, to investigate the properties of monomers of dimeric ribulose bisphosphate carboxylase/oxygenase. At temperatures below -5 degrees C or pressures above 1.5 kbar, only a diffuse band with low electrophoretic mobility was observed, which
An operator-induced conformational change in the C-terminal domain of the lambda repressor.
Journal:J Biol Chem
PubMed ID:1532575
'4,4''-bis(1-anilino-8-naphthalenesulfonic acid (Bis-ANS), an environment-sensitive fluorescent probe for hydrophobic region of proteins, binds specifically to the C-terminal domain of lambda repressor. The binding is characterized by positive cooperativity, the magnitude of which is dependent on protein concentration in the concentration range where dimeric repressor aggregates to a tetramer. In this
Exploiting new potential targets for anti-hepatitis B virus drugs.
Journal:Curr Drug Targets Infect Disord
PubMed ID:14529356
'Based on the recent studies of HBV strains with different replication efficiency, several new potential targets for anti-HBV replication have been presented. These include the viral and cellular regulatory factors associated with HBV replication and the process for encapsidation of viral genome and budding into endoplasmic reticulum (ER). A putative
Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach.
Journal:Biochemistry
PubMed ID:11467944
'Cysteine residues in tubulin are actively involved in regulating ligand interactions and microtubule formation both in vivo and in vitro. These cysteine residues are sensitive reporters in determining the conformation of tubulin. Although some of the cysteines are critical in modulating drug binding and microtubule assembly, it is not clear