Use Prolong Diamond with any Fluorescent Dye or Fluorescent Protein for brightest images. Learn more.

X-GlcU, CHA (5-Bromo-4-Chloro-3-Indolyl β-D-Glucuronide, Cyclohexylammonium Salt)

Catalog number: B1691

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

X-GlcU is a widely used chromogenic substrate for β-glucuronidase (GUS). It yields a dark blue precipitate at the site of enzymatic activity. X-GlcU is routinely used to detect GUS expression in transformed plant cells and tissues. X-GlcU can also be used to detect E. coli contamination in food and water.


• Chromogenic substrate
• Cell permeant
• Useful to detect GUS expression in transformed plant cells and tissues


Additional Information About Glycosidase Enzymes
Glycosidase enzymes exhibit very high selectivity for hydrolysis of their preferred sugars. For example, β-galactosidase rapidly hydrolyzes β-D-galactopyranosides but usually does not hydrolyze either the anomeric α-D-galactopyranosides or the isomeric β-D-glucopyranosides. Endogenous glycosidase activity is frequently used to characterize strains of microorganisms and to selectively label organelles of mammalian cells; defects in glycosidase activity are characteristic of several diseases.

In addition, glycosidases are important reporter gene markers. Specifically, lacZ, which encodes β-galactosidase, is extensively used as a reporter gene in animals and yeast, whereas the β-glucuronidase (GUS) gene is a popular reporter gene in plants. Glycosidase substrates are also used in conjunction with glycosidase-conjugated secondary detection reagents in immunohistochemical techniques and enzyme-linked immunosorbent assays (ELISAs). See all of our Molecular Probes® fluorogenic and chromogenic glycosidase substrates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability: Cell-Permeant
Detection Method: Colorimetric
Product Size: 100 mg
Substrate: X-GlcU
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Glucuronidase (GUS) Substrate
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols