BODIPY™ FL L-Cystine

L-cistina BODIPY™ FL

Número de catálogo	Cantidad
B20340	1 mg

Número de catálogo B20340

Precio (MXN)

Cantidad:

We have attached two BODIPY FL fluorophores to the amino groups of the disulfide-containing amino acid cystine to create this reagent for reversible thiol-specific labeling of thiolated oligonucleotides, proteins and cells. BODIPY FL L-cystine is virtually nonfluorescent due to interactions between the two fluorophores; however, thiol-specific exchange with thiolated biomolecules occurs to form mixed disulfides, resulting in green fluorescence.

Hemos adjuntado dos fluoróforos BODIPY FL a los grupos aminas de la cistina de aminoácidos que contienen disulfuro para crear este reactivo para el etiquetado reversible específico de tiol de oligonucleótidos tiolados, proteínas y células. La L-cistina BODIPY FL es prácticamente no fluorescente debido a las interacciones entre los dos fluoróforos; sin embargo, ocurre un intercambio específico de tiol con biomoléculas tioladas para formar disulfides mixtos, lo que resulta en una fluorescencia verde.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Reactividad químicaTiol

Etiqueta o tinteBODIPY™ FL

Tipo de productoL-cistina FL

Cantidad1 mg

Fracción reactivaTiol

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaColorantes BODIPY

Línea de productosBODIPY

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador (de – 5 a – 30 °C) y proteger de la luz.

Citations & References (2)

Citations & References

Abstract

Innate immune recognition triggers secretion of lysosomal enzymes by macrophages.

Authors:Lackman RL, Jamieson AM, Griffith JM, Geuze H, Cresswell P

Journal:Traffic

PubMed ID:17555533

'Gamma interferon-induced lysosomal thiolreductase (GILT) is expressed constitutively in antigen-presenting cells, where it reduces disulfide bonds to facilitate antigen presentation. GILT is synthesized as an enzymatically active precursor protein and is processed in early endosomes to yield the mature enzyme. The exposure of the promonocytic cell line THP-1 to Escherichia ... More

The radioprotective agent, amifostine, suppresses the reactivity of intralysosomal iron.

Authors:Yu Z, Eaton JW, Persson HL

Journal:Redox Rep

PubMed ID:14980067

Amifostine (2-[(3-aminopropyl)amino]ethane-thiol dihydrogen phosphate ester; WR-2721) is a radioprotective agent used clinically to minimize damage from radiation therapy to adjacent normal tissues. This inorganic thiophosphate requires dephosphorylation to produce the active, cell-permeant thiol metabolite, WR-1065. The activation step is presumably catalyzed by membrane-bound alkaline phosphatase, activity of which is substantially ... More