Regulation of the rate and extent of phospholipase C beta 2 effector activation by the beta gamma subunits of heterotrimeric G proteins.
AuthorsRunnels LW, Scarlata SF
JournalBiochemistry
PubMed ID9799521
'The activity of mammalian phosphoinositide-specific phospholipase C beta 2 (PLC-beta 2) is regulated by the alpha q family of G proteins and by beta gamma subunits. We measured the affinity between the laterally associating PLC-beta 2 and G beta gamma on membrane surfaces by fluorescence resonance energy transfer. Using a ... More
Quantitative analysis and characterization of biofunctionalized fluorescent silica particles.
AuthorsCorrie SR, Lawrie GA, Trau M
JournalLangmuir
PubMed ID16519476
A strategy for the production and subsequent characterization of biofunctionalized silica particles is presented. The particles were engineered to produce a bifunctional material capable of both (a) the attachment of fluorescent dyes for particle encoding and (b) the sequential modification of the surface of the particles to couple oligonucleotide probes. ... More
Runaway domain swapping in amyloid-like fibrils of T7 endonuclease I.
AuthorsGuo Z, Eisenberg D
JournalProc Natl Acad Sci U S A
PubMed ID16698921
Amyloid fibrils are associated with >20 fatal human disorders, including Alzheimer's, Parkinson's, and prion diseases. Knowledge of how soluble proteins assemble into amyloid fibrils remains elusive despite its potential usefulness for developing diagnostics and therapeutics. In at least some fibrils, runaway domain swapping has been proposed as a possible mechanism ... More
A genetically engineered, protein-based optical biosensor of myosin II regulatory light chain phosphorylation.
AuthorsPost PL, Trybus KM, Taylor DL
JournalJ Biol Chem
PubMed ID8175704
Myosin II is an important motor in the contraction of smooth and striated muscle as well as in a variety of non-muscle cell motile events including cytokinesis, cortical contractions during migration of fibroblasts, and capping of receptors. Phosphorylation of the 20-kDa light chain by myosin light chain kinase is part ... More
Heterogeneity of clara cell glutathione. A possible basis for differences in cellular responses to pulmonary cytotoxicants.
Clara-cell populations show a high degree of variation in susceptibility to injury by bioactivated cytotoxicants. Because glutathione (GSH) is critical for detoxification of electrophilic metabolites, heterogeneity in Clara cell GSH levels may lead to a wide range of cytotoxic responses. This study was designed to define the distinct GSH pools ... More
Development of a time-resolved fluorometric method for observing hybridization in living cells using fluorescence resonance energy transfer.
AuthorsTsuji A, Sato Y, Hirano M, Suga T, Koshimoto H, Taguchi T, Ohsuka S
JournalBiophys J
PubMed ID11423432
We previously showed that a specific kind of mRNA (c-fos) was detected in a living cell under a microscope by introducing two fluorescently labeled oligodeoxynucleotides, each labeled with donor or acceptor, into the cytoplasm, making them hybridize to adjacent locations on c-fos mRNA, and taking images of fluorescence resonance energy ... More