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Safranine O
Catalog number: B21674.09
10 g, Each
Thermo Scientific Chemicals
Safranine O
Catalog number: B21674.09
10 g, Each
Quantity
Catalog number: B21674.09
also known as B21674-09
Price (USD)
Price: 31.90
Online price: 27.65
Your price:
Quantity
-
Chemical Identifiers
CAS
477-73-6
IUPAC Name
2,7-diamino-1,8-dimethyl-5-phenyl-5λ⁵-phenazin-5-ylium chloride
Molecular Formula
C20H19ClN4
InChI Key
QRYAEWIQIBAZOJ-UHFFFAOYSA-N
SMILES
[Cl-].CC1=C(N)C=C2C(=C1)N=C1C(C)=C(N)C=CC1=[N+]2C1=CC=CC=C1
Specifications
Specification SheetAppearance (Color)
Red-brown or blue-green or dark green to black
Form
Powder or crystalline powder
Loss on Drying
≤15% (200?C/constant wt)
lambda-max (UV-Vis)
530-534nm (IMS: DI water, 1:1)
Specific Absorbance (E 1%/1cm)
≥1200 (IMS: DI water, 1:1)
Description
It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.
Solubility
Soluble in water at 20°C, 50g/L. Soluble in alcohol.
Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.
It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.
Solubility
Soluble in water at 20°C, 50g/L. Soluble in alcohol.
Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.
RUO – Research Use Only
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