Invitrogen™

BacLight™ Bacterial Membrane Potential Kit

Catalog number: B34950
Invitrogen™

BacLight™ Bacterial Membrane Potential Kit

Catalog number: B34950
Catalog Number
B34950
Unit Size
1 kit
Price (EUR)
Price: 423,00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (EUR)AvailabilityQuantity
B349501 kit
Price: 423,00
Your Price:
***
Product Overview
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The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Fluorescent
For Use With (Application)
Membrane Potential Assay
For Use With (Equipment)
Flow Cytometer
Product Type
Bacterial Membrane Potential Kit
Dye Type
Membrane Potential Probes
Emission
482⁄497
Format
Tube(s)
Product Line
BacLight™
Quantity
1 Kit
Shipping Condition
Wet Ice

Contents & Storage

Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light.