Invitrogen™

      BacLight™ Bacterial Membrane Potential Kit

      <i>Bac</i>Light™ Bacterial Membrane Potential Kit

      Catalog number:  B34950

       Related applications: Cell Viability, Proliferation & Function | Flow Cytometry | Microbiological Analysis

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      Description

      The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

      View additional information about all microbiology assays for flow cytometry.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Detection Method: Fluorescent
      Excitation⁄Emission (nm): 482⁄497
      Flow Cytometer Laser Lines: 488
      For Use With (Equipment): Flow Cytometer
      Indicator Properties: Fluorescent Dye-Based
      Indicator Type: Membrane Potential Probes
      Label or Dye: DiOC2(3)
      Number of Reactions: 100
      Product Line: BacLight™
      Product Size: 1 kit
      Shipping Condition: Wet Ice

      Contents & storage

      Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 µL of 500 µM solution in DMSO), and PBS (200 mL).

      Store in refrigerator (2–8°C) and protect from light.

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