Invitrogen™
BacLight™ Bacterial Membrane Potential Kit
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Description
The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Detection Method: | Fluorescent |
|---|---|
| Excitation⁄Emission (nm): | 482⁄497 |
| Flow Cytometer Laser Lines: | 488 |
| For Use With (Equipment): | Flow Cytometer |
| Indicator Properties: | Fluorescent Dye-Based |
| Indicator Type: | Membrane Potential Probes |
| Label or Dye: | DiOC2(3) |
| Number of Reactions: | 100 |
| Product Line: | BacLight™ |
| Product Size: | 1 kit |
| Shipping Condition: | Wet Ice |
Contents & storage
Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 µL of 500 µM solution in DMSO), and PBS (200 mL).
Store in refrigerator (2–8°C) and protect from light.
Store in refrigerator (2–8°C) and protect from light.
