BacLight™ Bacterial Membrane Potential Kit

Catalog number: B34950

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Flow Cytometry | Microbiological Analysis

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The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.


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Label or Dye: DiOC2(3)
Product Size: 1 kit
Indicator Type: Membrane Potential Probes
Detection Method: Fluorescent
Number of Reactions: 100
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Flow Cytometer
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 482⁄497
Shipping Condition: Wet Ice

Contents & storage

Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 µL of 500 µM solution in DMSO), and PBS (200 mL).

Store in refrigerator (2–8°C) and protect from light.


Manuals & protocols

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