The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Fluorescent
Dye Type
Membrane Potential Probes
Emission
482⁄497
Format
Tube(s)
Product Line
BacLight™
Quantity
1 Kit
Shipping Condition
Wet Ice
For Use With (Equipment)
Flow Cytometer
Product Type
Bacterial Membrane Potential Kit
Contents & Storage
Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 µM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light.
Figures
Detection of membrane potential in various bacteria with the BacLight™ Bacterial Membrane Potential Kit.
