Kit de potencial de la membrana bacteriana Bac Light™
Kit de potencial de la membrana bacteriana <i>Bac</i> Light&trade;
Citas y referencias (10)
Invitrogen™

Kit de potencial de la membrana bacteriana Bac Light™

El kit de potencial de la membrana bacteriana Bac Light™ el tinte indicador del potencial de la membrana fluorescente, DiOC2(3),Más información
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Número de catálogoCantidad
B349501 kit
Número de catálogo B34950
Precio (MXN)
-
Cantidad:
1 kit
El kit de potencial de la membrana bacteriana Bac Light™ el tinte indicador del potencial de la membrana fluorescente, DiOC2(3), junto con un protonófero (CCCP) y un tampón premezclado. El DiOC2(3) en concentraciones bajas muestra fluorescencia verde en todas las células bacterianas, pero se concentra más en células sanas que mantienen el potencial de la membrana, haciendo que el tinte se asocie y la emisión de fluorescencia cambie a rojo. Las poblaciones bacterianas rojas y verdes fluorescentes se distinguen fácilmente mediante un citómetro de flujo. El CCCP se incluye en el kit para su uso como control porque elimina el gradiente protónico, eliminando el potencial de membrana bacteriana.

Consulte información adicional sobre todos los ensayos microbiológicos para citometría de flujo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteSondas de potencial de membrana
FormatoTubo(s)
Cantidad1 kit
Condiciones de envíoHielo húmedo
Emission482⁄497
Para utilizar con (aplicación)Ensayo potencial de membrana
Para utilizar con (equipo)Citómetro de flujo
Línea de productosBacLight
Tipo de productoKit de potencial de membrana bacteriana
Unit SizeEach
Contenido y almacenamiento
Contiene DiOC2(3) (1,2 ml de solución de 3 mM en DMSO), CCCP (300 μl de solución de 500 μM en DMSO) y PBS (200 ml). Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (10)

Citations & References
Abstract
Membrane disruption by antimicrobial fatty acids releases low-molecular-weight proteins from Staphylococcus aureus.
Authors:Parsons JB, Yao J, Frank MW, Jackson P, Rock CO,
Journal:J Bacteriol
PubMed ID:22843840
The skin represents an important barrier for pathogens and is known to produce fatty acids that are toxic toward gram-positive bacteria. A screen of fatty acids as growth inhibitors of Staphylococcus aureus revealed structure-specific antibacterial activity. Fatty acids like oleate (18:1?9) were nontoxic, whereas palmitoleate (16:1?9) was a potent growth ... More
Comparative analysis of antimicrobial activities of valinomycin and cereulide, the Bacillus cereus emetic toxin.
Authors:Tempelaars MH, Rodrigues S, Abee T,
Journal:Appl Environ Microbiol
PubMed ID:21357430
'Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin produced by Streptomyces spp. and cereulide was ... More
Two heme-dependent terminal oxidases power Staphylococcus aureus organ-specific colonization of the vertebrate host.
Authors:Hammer ND, Reniere ML, Cassat JE, Zhang Y, Hirsch AO, Indriati Hood M, Skaar EP,
Journal:
PubMed ID:23900169
'Staphylococcus aureus is a significant cause of infections worldwide and is able to utilize aerobic respiration, anaerobic respiration, or fermentation as the means by which it generates the energy needed for proliferation. Aerobic respiration is supported by heme-dependent terminal oxidases that catalyze the final step of aerobic respiration, the reduction ... More
The biocide chlorine dioxide stimulates biofilm formation in Bacillus subtilis by activation of the histidine kinase KinC.
Authors:Shemesh M, Kolter R, Losick R,
Journal:J Bacteriol
PubMed ID:20971918
'Bacillus subtilis forms biofilms in response to signals that remain poorly defined. We report that biofilm formation is stimulated by sublethal doses of chlorine dioxide (ClO(2)), an extremely effective and fast-acting biocide. ClO(2) accelerated biofilm formation in B. subtilis as well as in other bacteria, suggesting that biofilm formation is ... More
Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.
Authors:Lyon DY, Alvarez PJ,
Journal:Environ Sci Technol
PubMed ID:19031913
Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular ... More
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