Invitrogen™

BacLight™ RedoxSensor™ CTC Vitality Kit

Catalog number: B34956
Have Questions?
Invitrogen™

BacLight™ RedoxSensor™ CTC Vitality Kit

Catalog number: B34956
Have Questions?
Catalog Number
B34956
Unit Size
1 kit
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
B349561 kit
-
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The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product. Cells not respiring or respiring at slower rates will reduce less CTC and consequently produce less fluorescent product, giving a semi-quantitative estimate of healthy vs. unhealthy bacteria. Green- and blue-fluorescent nucleic acid stains are included as counterstains to assist differentiation of cells from debris and calculating total cell numbers.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Bacteria
Detection Method
Fluorescence
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Product Type
CTC Vitality Kit
Dye Type
Other Label(s) or Dye(s)
Emission
488 nm
Format
Tube(s), Slide(s)
Product Line
BacLight™, RedoxSensor™
Quantity
1 Kit
Shipping Condition
Room Temperature
Solubility
DMSO (Dimethylsulfoxide)

Contents & Storage

• 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), 5 x 15 mg vials
• SYTO™ 24 green (100 μL in DMSO), 1 vial
• DAPI (5 mg/mL solution in water), 100 μL

Store in freezer (-5°C to -30°C) and protect from light.

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