BacLight™ RedoxSensor™ CTC Vitality Kit

Catalog number: B34956

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product. Cells not respiring or respiring at slower rates will reduce less CTC and consequently produce less fluorescent product, giving a semi-quantitative estimate of healthy vs. unhealthy bacteria. Green- and blue-fluorescent nucleic acid stains are included as counterstains to assist differentiation of cells from debris and calculating total cell numbers.

View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Compatible Cells: Bacteria
Detection Method: Fluorescent
Excitation⁄Emission (nm): CTC: 450⁄630, DAPI: 344⁄450, SYTO® 24: 490⁄515
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope
Format: Slide(s), Tube(s)
Label or Dye: CTC, SYTO® 24, DAPI
Number of Reactions: 50
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

Contains 5 vials of 5-cyano-2,3-ditolyl tetrazolium chloride (CTC, 15 mg per vial), 1 vial of SYTO® 24 green (100 µL in DMSO)m and DAPI (100 µL, 5 mg/mL solution in water).

Store in freezer (-5 to -30°C) and protect from light.

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Manuals & protocols

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