Kit de vitalidad CTC BacLight™ RedoxSensor™

Kit de vitalidad CTC <i>Bac</i>Light™ RedoxSensor™

Citas y referencias (6)

Invitrogen™

Kit de vitalidad CTC BacLight™ RedoxSensor™

El kit de vitalidad CTC BacLight™ RedoxSensor™ CTC proporciona reactivos eficaces para evaluar la salud y la vitalidad de lasMás información

Número de catálogo	Cantidad
B34956	1 Kit

Número de catálogo B34956

Precio (MXN)

Cantidad:

1 Kit

El kit de vitalidad CTC BacLight™ RedoxSensor™ CTC proporciona reactivos eficaces para evaluar la salud y la vitalidad de las células bacterianas que pueden resistir los procedimientos de fijación. En resumen, las células sanas que respiran a través de la cadena de transporte de electrones absorberán y reducirán el CTC en un producto de formazán fluorescente rojo insoluble. Las células que no respiran o respiran a velocidades más lentas reducirán menos CTC y, en consecuencia, producirán menos producto fluorescente, lo que dará una estimación semicuantitativa de bacterias sanas frente a enfermas. Las tinciones de ácido nucleico verde y azul fluorescente se incluyen como contratinciones para ayudar a diferenciar las células de los residuos y calcular el número total de células.

Consulte información adicional acerca de todos los ensayos microbiológicos para citometría de flujo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaBacterias

Método de detecciónFluorescente

Tipo de coloranteOtras etiquetas o colorantes

FormatoTubos, portaobjetos

Cantidad1 Kit

Condiciones de envíoTemperatura ambiente

SolubilidadDMSO (dimetilsulfóxido)

EmissionUV, 488

Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo

Línea de productosBacLight, RedoxSensor

Tipo de productoKit de vitalidad CTC

Unit SizeEach

Contenido y almacenamiento

Contiene cinco viales de cloruro de 5-ciano-2,3-ditolil tetrazolio (CTC, 15 mg por vial), un vial de SYTO™ 24 verde (100 µl en DMSO) m y DAPI (100 µl, 5 mg/ml de solución en agua).

Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (6)

Citations & References

Abstract

Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.

Authors:Sieracki ME, Cucci TL, Nicinski J,

Journal:Appl Environ Microbiol

PubMed ID:10347021

'The respiratory activity of marine bacteria is an important indication of the ecological functioning of these organisms in marine ecosystems. The redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is reduced intracellularly in respiring cells to an insoluble, fluorescent precipitate. This product is detectable and quantifiable by flow cytometry in individual cells. ... More

Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm.

Authors:Kim J, Pitts B, Stewart PS, Camper A, Yoon J,

Journal:Antimicrob Agents Chemother

PubMed ID:18195062

'The systematic understanding of how various antimicrobial agents are involved in controlling biofilms is essential in order to establish an effective strategy for biofilm control, since many antimicrobial agents are effective against planktonic cells but are ineffective when they are used against the same bacteria growing in a biofilm state. ... More

Characterization of osmotically induced filaments of Salmonella enterica.

Authors:Pratt ZL, Chen B, Czuprynski CJ, Wong AC, Kaspar CW,

Journal:Appl Environ Microbiol

PubMed ID:22798362

'Salmonella enterica forms aseptate filaments with multiple nucleoids when cultured in hyperosmotic conditions. These osmotic-induced filaments are viable and form single colonies on agar plates even though they contain multiple genomes and have the potential to divide into multiple daughter cells. Introducing filaments that are formed during osmotic stress into ... More

Inactivation of Escherichia coli by nanoparticulate zerovalent iron and ferrous ion.

Authors:Kim JY, Park HJ, Lee C, Nelson KL, Sedlak DL, Yoon J,

Journal:Appl Environ Microbiol

PubMed ID:20870787

'The mechanism of Escherichia coli inactivation by nanoparticulate zerovalent iron (nZVI) and Fe(II) was investigated using reactive oxygen species (ROS) quenchers and probes, an oxidative stress assay, and microscopic observations. Disruption of cell membrane integrity and respiratory activity was observed under deaerated conditions [more disruption by nZVI than Fe(II)], and ... More

How the insect immune system interacts with an obligate symbiotic bacterium.

Authors:Douglas AE, Bouvaine S, Russell RR,

Journal:Proc Biol Sci

PubMed ID:20719775

The animal immune system provides defence against microbial infection, and the evolution of certain animal-microbial symbioses is predicted to involve adaptive changes in the host immune system to accommodate the microbial partner. For example, the reduced humoral immune system in the pea aphid Acyrthosiphon pisum, including an apparently non-functional immune ... More

View all Kit de vitalidad CTC BacLight™ RedoxSensor™ citations