Invitrogen™

Biotin XX Tyramide SuperBoost™ Kit, Streptavidin

Catalog number:  B40931

Related applications:

Secondary Detection

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Description

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features biotin XX tyramide with HRP-conjugated streptavidin. For detection and additional signal amplification, conjugated streptavidin is used to detect specifically deposited biotin XX .

Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Product Line: SuperBoost™
Label or Dye: Biotin-XX

Contents & storage

1 kit sufficient for 150 microscope slides (18 x 18 mm), containing:

• Blocking buffer (1 X), 22.5 mL
• HRP-conjugated streptavidin (1 X), 22.5 mL
• Biotin-XX tyramide reagent
• Hydrogen peroxide (stabilized 3% solution), 28.5 mL
• Reaction buffer (20 X), 6 mL
• Reaction stop solution, 8 mg
• Dimethylsulfoxide (DMSO), 200 µL

Store (at 2°C to 8°C. When stored as directed, the product is stable for 6 months after receiving.)