These tyramide reagents are used in tyramide signal amplification (TSA), also called catalyzed reporter deposition (CARD). TSA is used to amplify signal up to 200-fold versus standard imaging methods. These reagents are provided stand-alone, but are also available as part of our SuperBoost™ tyramide signal amplification kits (available separately). They can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets. Up to seven-plex is possible in multiplex fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH).

Features include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO-3, secondary antibodies, and other SuperBoost kits
• Requires 10–100 times less primary antibody then standard ICC/IHC/ISH experiments

Tyramide reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. These reagents help sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. They are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a tyramide reagent can be imaged using any type of microscope, producing high-resolution multiplex images.

Dissolve the Alexa Fluor™ or Biotin-XX tyramide reagent in 150 μL of DMSO to prepare 100X stock. Tyramide may coat the sides of the vial so invert the vial several times to ensure all reagent is dissolved in solution.