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Tyramide Conjugates
Tyramide Conjugates
Invitrogen™

Tyramide Conjugates

Thermo Fisher Scientific bietet eine große Auswahl an hellen Alexa Fluor™, Alexa Fluor™ Plus oder Biotin XX Tyramid-Konjugaten. Diese Tyramid-Konjugate werden bei der Tyramid-Signalamplifikation (TSA), auch als katalysierte Reporterabscheidung (CARD, Catalyzed Reporter Deposition) bezeichnet, verwendet.
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KatalognummerKonjugatAnregung/Emission
B40958Alexa Fluor™ 647650/668 nm
B40952Alexa Fluor™ 350347/442 nm
B40953Alexa Fluor™ 488495/519 nm
B40954Alexa Fluor™ 546556/573 nm
B40955Alexa Fluor™ 555555/565 nm
B40956Alexa Fluor™ 568579/604 nm
B40957Alexa Fluor™ 594591/617 nm
B56131Alexa Fluor™ Plus 750750/790 nm
B40951Biotin-XX
Katalognummer B40958
Preis (EUR)
403,65
Exklusiv online
439,00
Ersparnis 35,35 (8%)
Each
Zum Warenkorb hinzufügen
Konjugat:
Alexa Fluor™ 647
Anregung/Emission:
650/668 nm
Preis (EUR)
403,65
Exklusiv online
439,00
Ersparnis 35,35 (8%)
Each
Zum Warenkorb hinzufügen
Thermo Fisher Scientific offers a wide variety of bright Alexa Fluor™, Alexa Fluor™ Plus or Biotin XX tyramide conjugates. These tyramide conjugates are used in tyramide signal amplification (TSA), also called catalyzed reporter deposition (CARD). TSA is used to amplify signals up to 200 fold versus standard imaging methods. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification to detect low abundance targets. Up to seven-plex is possible in multiplexable fluorescent imaging.

These tyramide reagents are used in tyramide signal amplification (TSA), also called catalyzed reporter deposition (CARD). TSA is used to amplify signal up to 200-fold versus standard imaging methods. These reagents are provided stand-alone, but are also available as part of our SuperBoost™ tyramide signal amplification kits (available separately). They can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets. Up to seven-plex is possible in multiplex fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH).

Features include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO-3, secondary antibodies, and other SuperBoost kits
• Requires 10–100 times less primary antibody then standard ICC/IHC/ISH experiments

Tyramide reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. These reagents help sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. They are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a tyramide reagent can be imaged using any type of microscope, producing high-resolution multiplex images.

Dissolve the Alexa Fluor™ or Biotin-XX tyramide reagent in 150 μL of DMSO to prepare 100X stock. Tyramide may coat the sides of the vial so invert the vial several times to ensure all reagent is dissolved in solution.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
KonjugatAlexa Fluor™ 647
NachweisverfahrenFluoreszenz
Anregung/Emission650/668 nm
VersandbedingungZugelassen für den Versand bei Raumtemperatur oder auf nassem Eis
ClonalityKeine
Zur Verwendung mit (Anwendung)IHC, BEI, ICC, ISH
ProduktlinieAlexa Fluor
Menge1 Fläschchen
Unit SizeEach
Inhalt und Lagerung
1 Fläschchen Alexa Fluor Tyramid-Reagenz, ausreichend für 150 Objektträger
Bei 2 °C bis 8 °C lagern. Bei vorschriftsmäßiger Lagerung ist dieses Produkt bis 6 Monate nach Erhalt stabil.)

Häufig gestellte Fragen (FAQ)

I used a neuron-specific antibody to label my neurons. I can't get enough signal from my fluorescent dye conjugated primary antibody. What can I do to improve it?

Here are our recommendations:

Use one of our extensive selection of secondary antibodies conjugated to bright, photostable Alexa Fluor dyes. The degree of labeling for each conjugate is 2-8 fluorophores per IgG molecule, with potentially three secondary antibody-binding sites per primary antibody, providing signal amplification of approximately 10-20 fluorophores per primary antibody.
Alternatively, primary antibody labeling can be detected with a biotinylated secondary antibody in conjunction with either a fluorescent streptavidin or a streptavidin bridge followed by a biotinylated reporter such as Qdot biotin. Although processing times increase with additional incubation and endogenous biotin-blocking steps, detection sensitivity also improves as a result of the labeled streptavidin.
For low-abundance targets, signal amplification may be necessary for optimal signal-to-noise ratios. Tyramide signal amplification (TSA) is an enzyme-mediated detection method that utilizes the catalytic activity of horseradish peroxidase (HRP) to generate reactive fluorophore-labeled tyramide radicals. These short-lived tyramide radicals covalently couple to nearby residues, producing an amplified fluorescent signal localized at the HRP-target interaction site.
For improved detection sensitivity with rapidly bleaching dyes, our SlowFade Diamond or ProLong Diamond antifade reagents have been shown to increase photostability and reduce initial fluorescence quenching in fixed cells, fixed tissues, and cell-free preparations.
Please review this web page for further optimization tips (https://www.thermofisher.com/us/en/home/references/newsletters-and-journals/bioprobes-journal-of-cell-biology-applications/bioprobes-issues-2011/bioprobes-66-october-2011/guide-to-immunocytochemistry.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have a very low-abundance antigen. How can I amplify my signal?

A common method for amplifying antibody detection is biotin-streptavidin detection, where a biotinylated secondary antibody is combined with subsequent labeling with a dye-conjugated streptavidin. This will amplify the signal by approximately 2-8 times, but endogenous biotin must be blocked beforehand. Another option is to use tyramide-signal amplification, where a horseradish peroxidase conjugate is used with a dye-labeled tyramide. This will amplify the signal by approximately 10-20 times, but endogenous peroxidase will need to be blocked. A final option may be to use a Qdot nanoparticle antibody or streptavidin conjugate, which can yield a signal as much as 40 times higher than a standard organic dye conjugate, depending on the Qdot color.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.