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Citas y referencias (18)
Invitrogen™
BTC, AM, cell permeant
BTC, AM es un indicador de Ca2+ basado en benzotiazol de cumarina que penetra en la célula. Muestra un cambioMás información
| Número de catálogo | Cantidad |
|---|---|
| B6791 | 100 μg |
Número de catálogo B6791
Precio (MXN)
-
Cantidad:
100 μg
BTC, AM es un indicador de Ca2+ basado en benzotiazol de cumarina que penetra en la célula. Muestra un cambio en la excitación máxima de entre aproximadamente 480 nm y 400 nm al unir Ca2+, lo que permite mediciones radiométricas esencialmente independientes del grosor desigual de la célula de carga de colorante, fotoblanqueo y fuga de colorante. El BTC tiene una alta selectividad para Ca2+ y Kd ∼7 µM, lo que permite la medición de niveles de Ca2+ intracelulares elevados.
Obtenga más información sobre los indicadores potenciales de iones, incluidos los indicadores de calcio, potasio, pH y de membrana ›
Obtenga más información sobre los indicadores potenciales de iones, incluidos los indicadores de calcio, potasio, pH y de membrana ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Cantidad100 μg
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Viabilidad y proliferación celulares
Para utilizar con (equipo)Citómetro de flujo
Tipo de productoIndicador de zinc
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (18)
Citations & References
Abstract
AM-loading of fluorescent Ca2+ indicators into intact single fibers of frog muscle.
Journal:Biophys J
PubMed ID:9168048
'The AM loading of a number of different fluorescent Ca2+ indicators was compared in intact single fibers of frog muscle. Among the 13 indicators studied, loading rates (the average increase in the fiber concentration of indicator per first 60 min of loading) varied approximately 100-fold, from approximately 3 microM/h to
Significance of Na/Ca exchange for Ca2+ buffering and electrical activity in mouse pancreatic beta-cells.
Journal:Biophys J
PubMed ID:10096898
'We have combined the patch-clamp technique with microfluorimetry of the cytoplasmic Ca2+ concentration ([Ca2+]i) to characterize Na/Ca exchange in mouse beta-cells and to determine its importance for [Ca2+]i buffering and shaping of glucose-induced electrical activity. The exchanger contributes to Ca2+ removal at [Ca2+]i above 1 microM, where it accounts for
Photolysis-induced suppression of inhibition in rat hippocampal CA1 pyramidal neurons.
Journal:J Physiol
PubMed ID:11410632
'1. Whole cell patch clamp recording, Ca(2+) measurement with ratiometric fluorescent dyes and photolysis of caged Ca(2+) were combined to investigate the depolarization- and photolysis-induced suppression of inhibition (DSI and PSI) in rat hippocampal CA1 pyramidal cells. 2. A 5-s depolarization from -70 mV to 0 mV or a 6-s
Ionized intracellular calcium concentration predicts excitotoxic neuronal death: observations with low-affinity fluorescent calcium indicators.
Journal:J Neurosci
PubMed ID:9254679
'Cytosolic calcium ([Ca2+]i) is an important mediator of neuronal signal transduction, participating in diverse biochemical reactions that elicit changes in synaptic efficacy, metabolic rate, and gene transcription. Excessive [Ca2+]i also has been implicated as a cause of acute neuronal injury, although measurement of [Ca2+]i in living neurons by fluorescent calcium
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC.
Journal:Cell Calcium
PubMed ID:10756974
'Accurate measurement of elevated intracellular calcium levels requires indicators with low calcium affinity and high selectivity. We examined fluorescence spectral properties and ionic specificity of three low-affinity, ratiometric indicators structurally related to Fura-2: mag-Fura-2 (furaptra), Fura-2FF, and BTC. The indicators differed in respect to their excitation wavelengths, affinity for Ca2+