Bacteria Counting Kit, for flow cytometry

Catalog number: B7277

Molecular Probes™  Related applications: Flow Cytometry | Microbiological Analysis

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Description

The Bacteria Counting Kit accurately enumerates bacteria by flow cytometry. The kit includes the SYTO™ BC dye, which is also available separately (Cat no. S34855), a high-affinity nucleic acid stain that easily penetrates both gram-negative and gram-positive bacteria, producing an exceptionally bright green-fluorescent signal. The kit also includes a calibrated suspension of polystyrene microspheres. Signals from both the stained bacteria and the beads are easily detected in the green fluorescence channel and can be distinguished on a plot of forward scatter versus fluorescence. The density of the bacteria in the sample can be determined from the ratio of bacterial signals to microsphere signals in the cytogram.

View additional information about all microbiology assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Solution
Solubility: DMSO (Dimethylsulfoxide)
Bead Diameter: 6 µm
Calibration Type: Flow Cytometry Cell Counting Beads
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 480⁄500
Format: Tube(s)
Detection Method: Fluorescent
For Use With (Equipment): Flow Cytometer
Label or Dye: SYTO® BC bacterial stain
Product Size: 1 kit
Shipping Condition: Room Temperature
Number of Reactions: 100

Contents & storage

Contains 1 vial of SYTO® BC bacterial stain (100 µL, 1000x in DMSO), 1 vial microsphere standard (1 mL, 6 µm).

Store in refrigerator (2–8°C) and protect from light.

Documents

Manuals & protocols