Bacteria Counting Kit, for flow cytometry

Citations & References (4)

Invitrogen™

Bacteria Counting Kit, for flow cytometry

The Bacteria Counting Kit accurately enumerates bacteria by flow cytometry. The kit includes the SYTO™ BC dye, which is alsoRead more

Catalog Number	Quantity
B7277	1 kit

Catalog number B7277

Price (HKD)

2,840.00

Add to cart

Quantity:

1 kit

Price (HKD)

2,840.00

Add to cart

The Bacteria Counting Kit accurately enumerates bacteria by flow cytometry. The kit includes the SYTO™ BC dye, which is also available separately (Cat No. S34855), a high-affinity nucleic acid stain that easily penetrates both gram-negative and gram-positive bacteria, producing an exceptionally bright green-fluorescent signal. The kit also includes a calibrated suspension of polystyrene microspheres. Signals from both the stained bacteria and the beads are easily detected in the green fluorescence channel and can be distinguished on a plot of forward scatter versus fluorescence. The density of the bacteria in the sample can be determined from the ratio of bacterial signals to microsphere signals in the cytogram.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

View additional information about all microbiology assays for flow cytometry.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Diameter (Metric) Bead6 μm

For Use With (Application)Flow Cytometry

For Use With (Equipment)Flow Cytometer

IncludesBC Bacterial Stain, Microsphere Standard

Quantity1 kit

Sample TypeBacteria

Shipping ConditionRoom Temperature

Product TypeBacteria Counting Kit

Unit SizeEach

Contents & Storage

Contains 1 vial of SYTO™ BC bacterial stain (100 μL, 1000x in DMSO), 1 vial microsphere standard (1 mL, 6 μm).

Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

Is the SYTO BC bacteria stain from the Bacteria Counting Kit available as a standalone product?

Yes, the SYTO BC bacteria stain can be purchased separately (Cat. No. S34855).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When using the Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry, some cells seem to have both red and green signal. Are these cells dead or alive or dying?

The green dye in the kit will label all the cells as it is a cell-permeant nucleic acid stain. The red dye is not cell permeant, and will only stain the cells with compromised membranes (dead cells). Therefore, any cells with red signal will be considered dead. It is possible that you will have some cells that are only red, some that are red and green, and some that are only green. Sometimes the red dye will displace the green dye. In any case, any red cells are dead.

Also, the green dye emission may bleed through into the red channel. Do a single-color staining and examine under both green and red filter sets to determine the level of bleedthrough. To avoid this bleedthrough, use a lower concentration of dye, and, if possible, use narrow bandpass filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How can I count my bacteria by flow cytometry?

There are several options. We have two fluorescence based kits that are useful for bacterial counting: Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856) and Bacteria Counting kit, for flow cytometry (Cat. No. B7277). Another option is the Flow Cytometry Sub-micron Particle Size Reference Kit (Cat. No. F13839).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using counting beads to count cells, but I cannot find the beads on my scatter plots. What do I do?

The first thing to do is check your threshold and see if it is set on forward scatter. If so, the beads are probably being excluded by the threshold. Reducing the threshold setting should reveal your beads.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Bacteria Counting Kit, for flow cytometry FAQs

Citations & References (4)

Citations & References

Abstract

Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.

Authors:Lebaron P, Parthuisot N, Catala P

Journal:Appl Environ Microbiol

PubMed ID:9572943

'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More

The beta-catenin--TCF-1 pathway ensures CD4(+)CD8(+) thymocyte survival.

Authors:Ioannidis V, Beermann F, Clevers H, Held W

Journal:Nat Immunol

PubMed ID:11477404

The association of trans-acting T cell factors (TCFs) or lymphoid enhancer factor 1 (LEF-1) with their coactivator beta-catenin mediates transient transcriptional responses to extracellular Wnt signals. We show here that T cell maturation depends on the presence of the beta-catenin--binding domain in TCF-1. This domain is necessary to mediate the ... More

Fluorescence staining and flow cytometry for monitoring microbial cells.

Authors:Veal DA, Deere D, Ferrari B, Piper J, Attfield PV

Journal:J Immunol Methods

PubMed ID:10986415

Large numbers of microbiological samples are analysed annually using traditional culture-based techniques. These techniques take hours to days to yield a result, are tedious and are not suitable for non-culturable microorganisms. Further, culture-based techniques do not provide real-time information on the physiological status of the organism in situ which is ... More

Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

Authors:Davey HM, Kell DB

Journal:Microbiol Rev

PubMed ID:8987359

The most fundamental questions such as whether a cell is alive, in the sense of being able to divide or to form a colony, may sometimes be very hard to answer, since even axenic microbial cultures are extremely heterogeneous. Analyses that seek to correlate such things as viability, which is ... More