High Five™ Cells in Express Five® Medium

Catalog number: B85502

Invitrogen™  Related applications: Insect Cell Culture | Protein Expression | Viral Vaccine Production

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Description

High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS). High Five™ Insect Cells are adapted to serum-free culture in Express Five® SFM for maximum cell growth and recombinant gene expression. High Five™ Insect Cells (frozen in Express Five® SFM) features:

• Recombinant protein expression from a variety of expression systems
• Up to ten-fold increase in secreted protein expression compared to Sf9 cells
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in High Five™ cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Up to ten-fold increase in secreted protein expression compared to Sf9 cells
High Five™ cells are the best choice for expression of secreted protein in insect cells. They consistently express 5–10 times more protein that Sf9 cells.

Good growth in adherent or suspension culture
High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Protocols for adherent growth can be found in Express Five® SFM are available in the product manual. High Five™ cells can be adapted to suspension culture.

Quality and performance testing
Each lot of High Five ™ cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme analysis.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

Specifications

Cell Line: High Five™
Media Adaptation: Express Five® SFM (Serum-Free Media)
Product Size: 3 x 106 cells
Species: Trichoplusia ni

Contents & storage

HIGH FIVE™ FROZEN CELLS:
• Storage conditions: liquid nitrogen (vapor phase)

EXPRESS FIVE™ Serum-free Medium:
• Storage conditions: 2°–8°C
• Protect from light

L GLUTAMINE, 100X:
• Storage conditions: -5° to 20°C
• Protect from light

Documents

Manuals & protocols

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