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Human IL-1 beta ELISA Kit, High Sensitivity - FAQs

Human IL-1 beta ELISA Kit, High Sensitivity - FAQs

View additional product information for Human IL-1 beta ELISA Kit, High Sensitivity - FAQs (BMS224HS)

6 product FAQs found

Will IL-1 beta Human ELISA Kit, High Sensitivity (Cat. No. BMS224HS) detect both total and active IL1 beta?

The antibody pair in IL-1 beta Human ELISA Kit, High Sensitivity is designed to recognize the active form of Human IL-1 beta. The level of cross-reactivity for the inactive form has not been determined.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

How should I prepare the standards for use with the High Sensitivity ELISA Kits?

We recommend that you prepare the standard in Eppendorf tubes.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Are the wash steps necessary when using your eBioscience High Sensitivity ELISA Kits?

Yes, please follow the washing steps exactly as outlined in the product manual (for optimal readings: an automatic plate washer is recommended).

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

For the eBioscience High Sensitivity ELISA Kits, can I perform the incubations at higher temperatures?

Incubations should occur at ambient temperatures not higher than 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

For the eBioscience High Sensitivity ELISA Kits, can I deviate from the recommended incubation times?

No, after adding the amplification reagents to the sample wells, the incubation times have to be followed exactly for optimal use.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

For the eBioscience High Sensitivity ELISA Kits, can the amplification reagents be prepared ahead of time?

No, the amplification reagents have to be prepared immediately before use. The reagent AR1 contains methanol which evaporates easily.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.