Invitrogen™

NativePAGE™ 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels

Catalog number: BN1004BOX
Invitrogen™

NativePAGE™ 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gels

Catalog number: BN1004BOX
The Invitrogen NativePAGE Bis-Tris Gel System is a precast polyacrylamide mini-gel system that provides sensitive, high-resolution analysis of native proteins and protein complexes for molecular mass estimations, and assessment of purity. It is based on the blue native polyacrylamide gel electrophoresis (BN PAGE) technique developed by Schägger and von Jagow, which overcomes the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility.
 
Catalog Number
BN1004BOX
Unit Size
10 gels (1 box)
Wells
15-well
Price (USD)
Price: 224.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 16%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsNative
Sample Loading VolumeUp to 15 µL
Separation Range30 to 10,000 kd
Separation TypeNative
Shelf Life12 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineNativePAGE™
Quantity10 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
10-well
Price (USD)
Price: 224.00
Your Price:
Availability
***
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 16%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsNative
Sample Loading VolumeUp to 25 µL
Separation Range30 to 10,000 kd
Separation TypeNative
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNativePAGE™
Quantity10 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeWellsPrice (USD)AvailabilityQuantity
BN1004BOXFull specifications
10 gels (1 box)15-well
Price: 224.00
Your Price:
***
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 16%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsNative
Sample Loading VolumeUp to 15 µL
Separation Range30 to 10,000 kd
Separation TypeNative
Shelf Life12 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineNativePAGE™
Quantity10 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
BN1002BOXFull specifications
10 gels (1 box)10-well
Price: 224.00
Your Price:
***
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 16%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsNative
Sample Loading VolumeUp to 25 µL
Separation Range30 to 10,000 kd
Separation TypeNative
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNativePAGE™
Quantity10 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 2 of 2

Features of the NativePAGE Bis-Tris Gel System include:
Wide molecular weight resolving range, from 15 – 10,000 kDa
Neutral-pH separation, which better preserves the native state of protein complexes
Resolution of all proteins in the gel regardless of their isoelectric point (pI)
Ability to analyze membrane-protein complexes in their native conformations
Ability to obtain better resolution than with traditional tris-glycine native electrophoresis

How NativePAGE Bis-Tris Gels work
In SDS-PAGE, SDS functions as a charge-shift molecule that denatures proteins by conferring on them a net negative charge and enables proteins to migrate towards the anode. BN PAGE uses Coomassie G-250 as the charge-shift molecule, which binds to proteins and confers a net negative charge while maintaining the proteins in their nondenatured, native state. The near-neutral pH of the NativePAGE Bis-Tris Gel System provides maximum stability of both the proteins and the gel matrix, resulting in a highly sensitive method for analysis of native membrane-protein complexes and offering superior band resolution over traditional Tris-glycine gel systems.

For transferring proteins to a membrane, we recommend using NuPAGE Transfer Buffers for traditional wet transfer and PVDF membranes. The NuPAGE transfer buffer maintains the neutral pH environment established during electrophoresis. Nitrocellulose membranes are not compatible for blotting since the membrane will bind the Coomassie G-250 dye very tightly. Alternatively, Rapid semi-dry transfer can be done using the Invitrogen Power Blotter (PB0013) or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001) using PVDF membranes.

For Research Use Only. Not for use in diagnostic procedures.

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