NativePure™ Lentiviral Gateway® Vector Kit

Catalog number: BN3001

Invitrogen™  Related applications: Protein Assays and Analysis | Protein Purification & Isolation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The NativePure™ Lentiviral Expression and Affinity Purification System provides an efficient method for lentiviral delivery of your gene of interest into cells, and the purification and analysis of native protein complexes. The NativePure™ Lentiviral Gateway® Vectors allow N- or C- terminal fusion of your recombinant protein of interest to the capTEV± affinity purification tag.

The Lentiviral Expression and Affinity Purification System provides:
• Gateway® vectors designed to express your protein of interest in multiple expression systems
• Lentiviral vectors with the capTEV™ Tag that allow in vivo biotinylation of your protein of interest
• Streamlined purification protocols with Streptavidin Agarose

Contents:
The NativePure™ Lentiviral Expression and Affinity Purification System includes the NativePure™ Lentiviral Gateway® Vector Kit, the NativePure™ Lentiviral Expression Kit, and the NativePure™ Lentiviral Affinity Purification Kit. Each kit is also available separately.

The NativePure™ Lentiviral Gateway® Vector Kit consists of the pLenti6/capTEV™-NT-DEST1 (N-terminal vector), pLenti6/capTEV™-CT-DEST (C-terminal vector), and pLenti6/capTEV™-CT-GW/ARP2 (C-terminal) control vector.

The NativePure™ Lentiviral Expression Kit contains the NativePure™ Lentiviral Gateway® Vector Kit, the ViraPower™ Bsd Lentiviral Support Kit, 293FT Cell Line, One Shot® Stbl3™ Chemically Competent E. coli, and Gateway® LR Clonase® Enzyme Mix.
The NativePure™ Lentiviral Affinity Purification Kit contains the NativePure™ Lentiviral Gateway® Vector Kit, Streptavidin Agarose, NativePure™ 5X Lysis/Binding Buffer, NativePure™ 10X TEV Cleavage Buffer, 100 mM DTT (Dithiothreitol), 10% NP40, AcTEV™ Protease, NativePure™ Columns, and NativePure™ Concentrators.

Store the 293FT Cell Line in liquid nitrogen and the One Shot® Stbl3™ Chemically Competent E. coli at -80°C.

Store Streptavidin Agarose, 10% NP-40, NativePure± 5X Lysis/Binding Buffer, NativePure± 10X TEV Cleavage Buffer, Lipofectamine™ 2000, NativePure± Columns, and NativePure± Concentrators at +4°C.

Store the NativePure™ Lentiviral Gateway® Vector Kit, AcTEV± Protease, 100 mM DTT, Gateway® LR Clonase® Enzyme Mix, ViraPower™ packaging Mix, and Blasticidin at -20°C. All components are guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Key Function: Viral Expression, Protein-Protein Interaction Studies
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag, BioEase Tag
Delivery Method: Lentiviral
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pLenti
Cleavage: TEV Protease Recognition Site
Product Size: 1 kit
Constitutive or Inducible System: Constitutive

Contents & storage

Store at room temperature.

Documents

Manuals & protocols