HCEC-3 500K CELLS⁄VIAL - FAQs

What media do you recommend for culturing Human Corneal Epithelial Cells (HCEC)?

We recommend culturing HCECs in keratinocyte serum-free medium (KSFM), as the cells are manufactured and quality-controlled using this medium. However, if a defined growth system is preferred, we recommend defined keratinocyte serum-free medium (Defined Keratinocyte-SFM). Note that a Coating Matrix Kit is required for culturing HCECs in Defined Keratinocyte-SFM. In-house testing has shown comparable HCEC growth and morphology in both KSFM and Defined Keratinocyte-SFM. Please note that these cells have been discontinued.