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Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488, 594, and 647 Azides This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

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The Click-iT® EdU Imaging Kit is a trial version of our superior alternative to traditional proliferation assays optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol. This trial-size kit provides sufficient EdU to label up to 6–30 coverslips and enough Alexa Fluor® 488, 594, and 647 azide to detect new DNA synthesis on 2–10 coverslips with each fluorophore.
• Simple—works the first time, every time, in less time
• Efficient—no denaturation steps or harsh treatment required
• Content-rich results—better preservation of cell morphology, antigen structure, and DNA integrity
• Consistent—not dependent on variable antibody lots for detection

The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA, with bromodeoxyuridine (BrdU) a commonly used analogue. BrdU-labeled DNA is quantitated using anti-BrdU antibodies following DNA denaturation by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules. This step is time consuming and difficult to perform consistently. The harsh treatment can also adversely effect sample integrity and quality, which makes co-staining with other antibodies challenging.

Superior Proliferation Methodology
The Click-iT® EdU Imaging Kit provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT® EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT® EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT® EdU Imaging Kit is not only easy to use, but more accurate and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.

This kit is optimized for fluorescence microscopy applications; visit the Click-iT® technology area of our website for kits designed for high-content imaging, fluorescence microplate, or flow cytometry platforms.

Learn more about Click-iT® technology

The Click-iT® assay can be used on cells in culture or in vivo following EdU administration by feeding or injection methods.
The Click-iT® assay can be used with BrdU in dual pulse experiments by using the anti-BrdU (clone MoBu-1) antibody, which does not cross react with EdU.
The Click-iT® technology is compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed cell labeling.
For Research Use Only. Not for use in diagnostic procedures.


Compatible Cells: Mammalian Cells, Sperm Cells, Fungal Cells, Eukaryotic Cells, Bacteria, Yeast Cells
Emission: Visible
Excitation: Visible
Excitation/Emission: 495⁄519, 650/665 (Far-Red), 590/617 (Red)
Product Line: Alexa Fluor™, Click-iT™
Reaction Time: 70 min
Shipping Condition: Room Temperature
Detection Method: Fluorescent
For Use With (Equipment): Floid® Cell Imaging System, Fluorescence Microscope, Fluorescent Imager
Label Type: Alexa Fluor Dyes, Click Chemistry Label
Label or Dye: Alexa Fluor™ 488, Alexa Fluor™ 594, Alexa Fluor™ 647
Format: Coverslip(s)
Color: Green, Far-Red, Red

Contents & storage

Store in refrigerator (2–8°C).