Invitrogen™

Click-IT™ AHA (L-Azidohomoalanine)

Catalog number: C10102
Have Questions?
Invitrogen™

Click-IT™ AHA (L-Azidohomoalanine)

Catalog number: C10102
Have Questions?
Catalog Number
C10102
Unit Size
5 MG
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C101025 MG
-
Product Overview
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Click-iT® AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT® Protein Analysis Detection Kits containing either TAMRA, Dapoxyl®, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis or Multiplexed Proteomics® reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Green Features
Less hazardous
Product Type
AHA
Quantity
5 mg
Detection Method
Biotin-Based, Fluorescent
Labeling Method
Metabolic Labeling
Shipping Condition
Room Temperature
Product Line
Click-iT™, Molecular Probes™
Label or Dye
Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)
Labeling Target
Proteins

Contents & Storage

Store in freezer -5°C to -30°C.

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