Invitrogen™

Click-IT™ AHA (L-Azidohomoalanine) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  C10102

Related applications:

Protein Biology

| Cell Analysis

Back to top

Description

Click-iT® AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT® Protein Analysis Detection Kits containing either TAMRA, Dapoxyl®, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis or Multiplexed Proteomics® reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Labeling Method: Metabolic Labeling
Labeling Target: Proteins (Nascent)
Shipping Condition: Room Temperature
Product Line: Click-iT™, Molecular Probes®
Detection Method: Biotin-Based, Fluorescent
Label or Dye: Alexa Fluor™ 488, Alexa Fluor™ 555, Alexa Fluor™ 594, Alexa Fluor™ 647, Biotin, Oregon Green® 488, TMR (Tetramethylrhodamine)
Green Features: Less hazardous

Contents & storage

Store in freezer (-5 to -30°C).