Invitrogen™

Click-IT™ AHA (L-Azidohomoalanine)

Green features
Catalog number: C10102
Invitrogen™

Click-IT™ AHA (L-Azidohomoalanine)

Green features
Catalog number: C10102
Catalog Number
C10102
Unit Size
5 MG
Price (USD)
323.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C101025 MG
323.00
Your Price:
***
Product Overview
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Additional Information
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Click-iT® AHA (L-azidohomoalaine) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional radioactive technique, 35S-methionine for the detection of nascent protein. AHA is an amino acid analog that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click " reaction between an azide and an alkyne where the azido modified protein is detected with one of the Click-iT® Protein Analysis Detection Kits containing either TAMRA, Dapoxyl®, or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis or Multiplexed Proteomics® reagents for differential analyses of newly synthesized proteins together with total glycoproteins, total phosphoproteins or total protein.

Specifications

Green Features
Less hazardous
Product Type
AHA
Quantity
5 mg
Detection Method
Biotin-Based, Fluorescent
Labeling Method
Metabolic Labeling
Shipping Condition
Room Temperature
Product Line
Click-iT™, Molecular Probes™
Label or Dye
Alexa Fluor™ 488, 555, 594, 647, Biotin, Oregon Green™ 488, TMR (Tetramethylrhodamine)
Labeling Target
Proteins (Nascent)

Contents & Storage

Store in freezer -5°C to -30°C.

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