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Countess™ Cell Counting Chamber Slides with Trypan Blue Stain, disposable, 50 Slides, 2 x 1 mL vials - FAQs

Countess™ Cell Counting Chamber Slides with Trypan Blue Stain, disposable, 50 Slides, 2 x 1 mL vials - FAQs

View additional product information for Countess™ Cell Counting Chamber Slides - FAQs (A40008469, C10283, C10315, C10314, A25750, C10228, C10313, C10312)

10 product FAQs found

Do you offer an alternative for Countess Cell Counting Chamber Slides (Cat. no. C10283) for Countess 3 and Countess 3 FL Automated Cell Counter?

Yes. The alternative options for Countess Cell Counting Chamber Slides (Cat. No. C10283) and for Countess 3 and Countess 3 FL Automated Cell Counter can be found using the link below:

Countess Cell Counting Chamber Slides (Cat. Nos. C10228, C10312, C10313, C10314, C10315)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What Countess Automated Cell Counter instruments are compatible with the Countess Cell Counting Chamber Slides (Cat. No. C10228, C10312, C10314, C10315)?

The Countess Cell Counting Chamber Slides (Cat. No. C10228, C10312, C10314, C10315) are compatible with all Countess Automated Cell Counter instruments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center .

How often do I have to calibrate the Invitrogen Countess II and Invitrogen Countess II FL instruments?

The Countess II and Countess II FL instruments come precalibrated. You do not have to calibrate the instruments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do the Invitrogen Countess II and InvitrogenCountess II FL instruments distinguish live and dead cells?

It is based on dye exclusion using 0.2% Trypan Blue dye. Trypan Blue is excluded by the live cells and enters the dead cells; this results in intense staining of the intracellular proteins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What cell types have been tested with the Invitrogen Countess II and Invitrogen Countess II FL instruments?

The following cell lines and primary cells have been tested:

- Cell lines: HEK 293, A431, CHO-M1, CHSE, COLO-205, COS-7, HeLa, HepG2, HL-60, J774(MMM), Jurkat, K-562, MCF-7, MRC-5, NIH/3T3, PC-12, SF-21, U266, and U2OS

- Primary cells: Human adipose tissue-derived stem cells, HASMCs, HPAECs, HPASMCs, HUVECs, C2C12, RBCs, and PMBCs

We are continually testing additional cell types.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What count-to-count precision should be expected with the Invitrogen Countess II and Invitrogen Countess II FL instruments?

When an experienced worker manually counts cells using a glass hemocytometer together with a microscope, count-to-count variability of a single sample is commonly 10% or more. Users of Countess II and Countess II FL instruments should typically see <5% count-to-count variability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the Invitrogen Countess II and Invitrogen Countess II FL instruments, why do my brightfield and fluorescent counts differ by 2x?

When counting in brightfield mode, the Countess II and Countess II FL instruments automatically assume a 1:1 dilution has been made with trypan blue. The same assumption is not made when in fluorescence mode.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the counting range with Invitrogen Countess II instruments?

The Countess II instruments are designed to read samples with concentrations in the range of 1 x 10E4 cells/mL to 1 x 10E7 cells/mL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long do the Invitrogen Countess II and Invitrogen Countess II FL instruments take to count cells?

The time taken is ˜10 seconds per sample.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size of cells can the Invitrogen Countess II Automated Cell Counter and Invitrogen Countess II FL Automated Cell Counter count?

The Countess II and Countess II FL instruments can detect particles/cells ranging from 4 - 60 µm. Cells ranging from 7 - 60 µm can be counted and assessed for viability with brightfield viewing.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.