Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.
AuthorsLiu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H,
JournalGenes Cells
PubMed ID20236182
'DEC1 (BHLHB2/Stra13/Sharp2) and DEC2 (BHLHB3/Sharp1) are basic helix-loop-helix (bHLH) transcription factors that are involved in circadian rhythms, differentiation and the responses to hypoxia. We examined whether DEC1 and DEC2 are involved in apoptosis regulation, in human breast cancer MCF-7 cells. We found that siRNA-mediated knockdown of DEC2 resulted in marked ... More
Soluble amyloid beta-protein dimers isolated from Alzheimer cortex directly induce Tau hyperphosphorylation and neuritic degeneration.
AuthorsJin M, Shepardson N, Yang T, Chen G, Walsh D, Selkoe DJ,
JournalProc Natl Acad Sci U S A
PubMed ID21421841
'Alzheimer disease is a major cause of cognitive failure, and a pathogenically related but more subtle process accounts for many cases of mild memory symptoms in older humans. Insoluble fibrillar plaques of amyloid ß-proteins (Aß) and neurofibrillary deposits of hyperphosphorylated tau proteins are the diagnostic lesions of AD, but their ... More
Directional neuronal migration is impaired in mice lacking adenomatous polyposis coli 2.
AuthorsShintani T, Takeuchi Y, Fujikawa A, Noda M,
JournalJ Neurosci
PubMed ID22573669
'Adenomatous polyposis coli 2 (APC2) is a family member of APC and mainly expressed in the nervous system. We previously reported that APC2 plays a critical role in axonal projection through the regulation of microtubule stability. Here, we show that a lack of Apc2 induces severe laminary defects in some ... More
P2X7-mediated calcium influx triggers a sustained, PI3K-dependent increase in metabolic acid production by osteoblast-like cells.
AuthorsGrol MW, Zelner I, Dixon SJ,
JournalAm J Physiol Endocrinol Metab
PubMed ID22185840
'The P2X7 receptor is an ATP-gated cation channel expressed by a number of cell types, including osteoblasts. Genetically modified mice with loss of P2X7 function exhibit altered bone formation. Moreover, activation of P2X7 in vitro stimulates osteoblast differentiation and matrix mineralization, although the underlying mechanisms remain unclear. Because osteogenesis is ... More
Astrocytes secrete exosomes enriched with proapoptotic ceramide and prostate apoptosis response 4 (PAR-4): potential mechanism of apoptosis induction in Alzheimer disease (AD).
AuthorsWang G, Dinkins M, He Q, Zhu G, Poirier C, Campbell A, Mayer-Proschel M, Bieberich E,
JournalJ Biol Chem
PubMed ID22532571
'Amyloid protein is well known to induce neuronal cell death, whereas only little is known about its effect on astrocytes. We found that amyloid peptides activated caspase 3 and induced apoptosis in primary cultured astrocytes, which was prevented by caspase 3 inhibition. Apoptosis was also prevented by shRNA-mediated down-regulation of ... More
Nutrient excess stimulates ß-cell neogenesis in zebrafish.
AuthorsMaddison LA, Chen W,
JournalDiabetes
PubMed ID22721970
'Persistent nutrient excess results in a compensatory increase in the ß-cell number in mammals. It is unknown whether this response occurs in nonmammalian vertebrates, including zebrafish, a model for genetics and chemical genetics. We investigated the response of zebrafish ß-cells to nutrient excess and the underlying mechanisms by culturing transgenic ... More
G9a histone methyltransferase activity in retinal progenitors is essential for proper differentiation and survival of mouse retinal cells.
AuthorsKatoh K, Yamazaki R, Onishi A, Sanuki R, Furukawa T,
JournalJ Neurosci
PubMed ID23223288
'In vertebrate retinal development, various transcription factors are known to execute essential activities in gene regulation. Although epigenetic modification is considered to play a pivotal role in retinal development, the exact in vivo role of epigenetic regulation is still poorly understood. We observed that G9a histone methyltransferase, which methylates histone ... More
Divergent fate and origin of neurosphere-like bodies from different layers of the gut.
'Enteric neural stem cells (ENSCs) are a population of neural crest-derived multipotent stem cells present in postnatal gut that may play an important role in regeneration of the enteric nervous system. In most studies, these cells have been isolated from the layer of the gut containing the myenteric plexus. However, ... More
Cocaine- and amphetamine-regulated transcript (CART) protects beta cells against glucotoxicity and increases cell proliferation.
AuthorsSathanoori R, Olde B, Erlinge D, Göransson O, Wierup N,
JournalJ Biol Chem
PubMed ID23250745
'Cocaine- and amphetamine-regulated transcript (CART) is an islet peptide that promotes glucose-stimulated insulin secretion in beta cells via cAMP/PKA-dependent pathways. In addition, CART is a regulator of neuronal survival. In this study, we examined the effect of exogenous CART 55-102 on beta cell viability and dissected its signaling mechanisms. Evaluation ... More
Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis.
AuthorsDougherty M, Kamel G, Grimaldi M, Gfrerer L, Shubinets V, Ethier R, Hickey G, Cornell RA, Liao EC,
JournalDevelopment
PubMed ID23154410
Development of the palate in vertebrates involves cranial neural crest migration, convergence of facial prominences and extension of the cartilaginous framework. Dysregulation of palatogenesis results in orofacial clefts, which represent the most common structural birth defects. Detailed analysis of zebrafish palatogenesis revealed distinct mechanisms of palatal morphogenesis: extension, proliferation and ... More
?-Aminobutyric acid regulates both the survival and replication of human ß-cells.
AuthorsTian J, Dang H, Chen Z, Guan A, Jin Y, Atkinson MA, Kaufman DL,
Journal
PubMed ID23995958
?-Aminobutyric acid (GABA) has been shown to inhibit apoptosis of rodent ß-cells in vitro. In this study, we show that activation of GABAA receptors (GABAA-Rs) or GABAB-Rs significantly inhibits oxidative stress-related ß-cell apoptosis and preserves pancreatic ß-cells in streptozotocin-rendered hyperglycemic mice. Moreover, treatment with GABA, or a GABAA-R- or GABAB-R-specific ... More
Morphine epigenomically regulates behavior through alterations in histone H3 lysine 9 dimethylation in the nucleus accumbens.
AuthorsSun H, Maze I, Dietz DM, Scobie KN, Kennedy PJ, Damez-Werno D, Neve RL, Zachariou V, Shen L, Nestler EJ,
JournalJ Neurosci
PubMed ID23197736
Dysregulation of histone modifying enzymes has been associated with numerous psychiatric disorders. Alterations in G9a (Ehmt2), a histone methyltransferase that catalyzes the euchromatic dimethylation of histone H3 at lysine 9 (H3K9me2), has been implicated recently in mediating neural and behavioral plasticity in response to chronic cocaine administration. Here, we show ... More
Pannexin1 contributes to pathophysiological ATP release in lipoapoptosis induced by saturated free fatty acids in liver cells.
AuthorsXiao F, Waldrop SL, Khimji AK, Kilic G,
JournalAm J Physiol Cell Physiol
PubMed ID22972801
Hepatocyte lipoapoptosis induced by saturated free fatty acids (FFA) contributes to hepatic inflammation in lipotoxic liver injury, and the cellular mechanisms involved have not been defined. Recent studies have shown that apoptosis in nonhepatic cells stimulates ATP release via activation of pannexin1 (panx1), and extracellular ATP functions as a proinflammatory ... More
Sh3rf2/POSHER protein promotes cell survival by ring-mediated proteasomal degradation of the c-Jun N-terminal kinase scaffold POSH (Plenty of SH3s) protein.
We report that Sh3rf2, a homologue of the pro-apoptotic scaffold POSH (Plenty of SH3s), acts as an anti-apoptotic regulator for the c-Jun N-terminal kinase (JNK) pathway. siRNA-mediated knockdown of Sh3rf2 promotes apoptosis of neuronal PC12 cells, cultured cortical neurons, and C6 glioma cells. This death appears to result from activation ... More
Notch gain of function in mouse periocular mesenchyme downregulates FoxL2 and impairs eyelid levator muscle formation, leading to congenital blepharophimosis.
AuthorsZhang Y, Kao WW, Pelosi E, Schlessinger D, Liu CY,
JournalJ Cell Sci
PubMed ID21730020
Notch signaling is pivotal for the morphogenesis and homeostasis of many tissues. We found that aberrant Notch activation in mouse neural-crest-derived periocular mesenchymal cells (POMCs), which contribute to the formation of corneal and eyelid stroma, results in blepharophimosis. Compound transgenic mice overexpressing the Notch1 intracellular domain (N1-ICD) in POMCs (POMC(N1-ICD)) ... More
Requirement for invariant chain in macrophages for Mycobacterium tuberculosis replication and CD1d antigen presentation.
AuthorsSillé FC, Martin C, Jayaraman P, Rothchild A, Fortune S, Besra GS, Behar SM, Boes M,
JournalInfect Immun
PubMed ID21576321
Mycobacterium tuberculosis is an intracellular bacterium that persists in phagosomes of myeloid cells. M. tuberculosis-encoded factors support pathogen survival and reduce fusion of phagosomes with bactericidal lysosomal compartments. It is, however, not entirely understood if host factors that mediate endosomal fusion affect M. tuberculosis intracellular localization and survival. Neither is ... More
C-propeptides of procollagens I alpha 1 and II that differentially accumulate in enchondromas versus chondrosarcomas regulate tumor cell survival and migration.
Chondrogenic tumors that exhibit benign or malignant behaviors synthesize variable amounts of cartilage-like extracellular matrix. To define the regulators of these phenotypes, we performed a proteomic comparison of multiple human chondrogenic tumors, which revealed differential accumulation of the C-propeptides of procollagens Ialpha1 and II (PC1CP and PC2CP) in malignant versus ... More
Impact of Sustained Exposure to ß-amyloid on Calcium Homeostasis and Neuronal Integrity in a Model Nerve Cell System Expressing a4ß2 Nicotinic Acetylcholine Receptors.
AuthorsArora K, Alfulaij N, Higa JK, Panee J, Nichols RA,
JournalJ Biol Chem
PubMed ID23479730
Although the interaction between ß-amyloid (Aß) and nicotinic acetylcholine receptors has been widely studied, the impact of prolonged exposure to Aß on nAChR expression and signaling is not known. In this study, we employed a neuronal culture model to better understand the impact of sustained exposure of Aß on the ... More
Vasopressin inhibits apoptosis in renal collecting duct cells.
The peptide hormone arginine vasopressin (AVP) plays a critical role in regulating salt and water transport in the mammalian kidney. Recent studies have also demonstrated that AVP can promote cell survival in neuronal cells through V1 receptors. The current study addresses whether AVP can inhibit apoptosis in kidney collecting duct ... More
Promotion of airway anastomotic microvascular regeneration and alleviation of airway ischemia by deferoxamine nanoparticles.
Authors
JournalBiomaterials
PubMed ID24161166
Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform.
Authors
JournalElife
PubMed ID31451149
N-myc regulates growth and fiber cell differentiation in lens development.
AuthorsCavalheiro GR, Matos-Rodrigues GE, Zhao Y, Gomes AL, Anand D, Predes D, de Lima S, Abreu JG, Zheng D, Lachke SA, Cvekl A, Martins RAP
JournalDev Biol
PubMed ID28716713
'Myc proto-oncogenes regulate diverse cellular processes during development, but their roles during morphogenesis of specific tissues are not fully understood. We found that c-myc regulates cell proliferation in mouse lens development and previous genome-wide studies suggested functional roles for N-myc in developing lens. Here, we examined the role of N-myc ... More
Melatonin Attenuates Cardiac Reperfusion Stress by Improving OPA1-Related Mitochondrial Fusion in a Yap-Hippo Pathway-Dependent Manner.
AuthorsMa S, Dong Z
JournalJ Cardiovasc Pharmacol
PubMed ID30418242
'The role of OPA1-related mitochondrial fusion in cardiac reperfusion stress has remained elusive. The aim of our study is to explore whether melatonin alleviates cardiac ischemia-reperfusion (IR) injury by modulating OPA1-related mitochondrial fusion. We found that melatonin reduced infarct area, sustained myocardial function, and suppressed cardiomyocyte death during cardiac reperfusion ... More
Radiation Sensitivity in a Preclinical Mouse Model of Medulloblastoma Relies on the Function of the Intrinsic Apoptotic Pathway.
Authors
JournalCancer Res
PubMed ID27197166
Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation.
Authors
JournalCell Stem Cell
PubMed ID28803918
Nucleotidyl transferase assisted DNA labeling with different click chemistries.
Authors
JournalNucleic Acids Res
PubMed ID26013812
HIF-2α is essential for carotid body development and function.
Authors
JournalElife
PubMed ID29671738
Epigenetic therapy overcomes treatment resistance in T cell prolymphocytic leukemia.
Authors
JournalSci Transl Med
PubMed ID26109102
Developmental Role of Macrophage Cannabinoid-1 Receptor Signaling in Type 2 Diabetes.
Authors
JournalDiabetes
PubMed ID28082458
Bladder cancer therapy using a conformationally fluid tumoricidal peptide complex.
Authors
JournalNat Commun
PubMed ID34103518
The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels.
Authors
JournalNeuron
PubMed ID29154126
Local convection-enhanced delivery of an anti-CD40 agonistic monoclonal antibody induces antitumor effects in mouse glioma models.
AuthorsShoji T, Saito R, Chonan M, Shibahara I, Sato A, Kanamori M, Sonoda Y, Kondo T, Ishii N, Tominaga T
JournalNeuro Oncol
PubMed ID26917236
Glioblastoma is one of the most malignant brain tumors in adults and has a dismal prognosis. In a previous report, we reported that CD40, a TNF-R-related cell surface receptor, and its ligand CD40L were associated with glioma outcomes. Here we attempted to activate CD40 signaling in the tumor and determine ... More
Transcription factors Nrf2 and NF-?B contribute to inflammation and apoptosis induced by intestinal ischemia-reperfusion in mice.
AuthorsMeng QT, Chen R, Chen C, Su K, Li W, Tang LH, Liu HM, Xue R, Sun Q, Leng Y, Hou JB, Wu Y, Xia ZY
JournalInt J Mol Med
PubMed ID29039475
Intestinal ischemia/reperfusion (IIR) is a common pathological event associated with intestinal injury and apoptosis with high mortality. Nuclear factor (NF)-E2-related factor-2 (Nrf2) is a key transcription factor that interacts with NF-?B and has a vital anti-inflammatory effect. However, whether Nrf2 has a role in IIR-induced apoptosis and the possible underlining ... More
Alkaloids of fascaplysin are effective conventional chemotherapeutic drugs, inhibiting the proliferation of C6 glioma cells and causing their death
AuthorsBryukhovetskiy I, Lyakhova I, Mischenko P, Milkina E, Zaitsev S, Khotimchenko Y, Bryukhovetskiy A, Polevshchikov A, Kudryavtsev I, Khotimchenko M, Zhidkov M
JournalOncol Lett
PubMed ID28356953
Glioblastoma multiforme is an invasive malignant glial brain tumor with a poor prognosis for patients. The primary reasons that lead to the development of treatment resistance are associated with tumor cells infiltrating the brain parenchyma and the specific properties of tumor stem cells. A crucial research area in medical science ... More
Uremic Advanced Glycation End Products and Protein-Bound Solutes Induce Endothelial Dysfunction Through Suppression of Krüppel-Like Factor 2.
Cardiovascular disease is the leading cause of morbidity and mortality in patients with end-stage renal disease. The accumulation of uremic solutes in this patient population is associated with endothelial dysfunction and accelerated cardiovascular disease. In this study, we examined the impact of the uremic milieu on the endothelial transcription factor, ... More
Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat.
AuthorsEl-Boshy M, BaSalamah MA, Ahmad J, Idris S, Mahbub A, Abdelghany AH, Almaimani RA, Almasmoum H, Ghaith MM, Elzubier M, Refaat B
JournalFree Radic Biol Med
PubMed ID31255668
Acute paracetamol (APAP) toxicity is a leading cause of liver, and less commonly renal, injuries through oxidative stress and inflammation. Albeit vitamin D (VD) is a well-known anti-oxidant and anti-inflammatory hormone, there is no report on its potential protective/therapeutic actions against APAP acute toxicity. This study, therefore, measured the interplay ... More
The role of the fat mass and obesity-associated protein in the proliferation of pancreatic cancer cells.
AuthorsTang X, Liu S, Chen D, Zhao Z, Zhou J
JournalOncol Lett
PubMed ID30719115
Fat mass and obesity-associated (FTO) protein has been identified as a critical demethylase in regulating cellular mRNA stability by removing N
Beneficial Effects of Low Frequency Vibration on Human Chondrocytes in Vitro.
AuthorsLützenberg R, Wehland M, Solano K, Nassef MZ, Buken C, Melnik D, Bauer J, Kopp S, Krüger M, Riwaldt S, Hemmersbach R, Schulz H, Infanger M, Grimm D
JournalCell Physiol Biochem
PubMed ID31550089
In articular cartilage, chondrocytes are the predominant cell type. A long-term stay in space can lead to bone loss and cartilage breakdown. Due to the poor regenerative capacity of cartilage, this may impair the crewmembers' mobility and influence mission activities. Beside microgravity other factors such as cosmic radiation and vibration ... More
Controlled Heterotypic Pseudo-Islet Assembly of Human ß-Cells and Human Umbilical Vein Endothelial Cells Using Magnetic Levitation.
AuthorsUrbanczyk M, Zbinden A, Layland SL, Duffy G, Schenke-Layland K
JournalTissue Eng Part A
PubMed ID31680653
ß-Cell functionality and survival are highly dependent on the cells' microenvironment and cell-cell interactions. Since the pancreas is a highly vascularized organ, the crosstalk between ß-cells and endothelial cells (ECs) is vital to ensure proper function. To understand the interaction of pancreatic ß-cells with vascular ECs, we sought to investigate ... More