Invitrogen™

Click-iT™ RNA Alexa Fluor™ 488 HCS Assay

Green features
Catalog number: C10327
Invitrogen™

Click-iT™ RNA Alexa Fluor™ 488 HCS Assay

Green features
Catalog number: C10327
Catalog Number
C10327
Unit Size
1 kit
Price (USD)
598.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C103271 kit
598.00
Your Price:
***
Product Overview
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The Click-iT® RNA Alexa Fluor® 488 HCS Assay enables detection of global RNA transcription temporally and spatially in cells (PNAS (2008) 105:15779-84). The ability to detect newly synthesized RNA or changes in RNA levels resulting from disease, environmental damage or drug treatments is an important aspect of toxicological profiling. Utilizing an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry, newly synthesized RNA can be detected without the use of radioactivity or antibodies with a simple, two-step procedure. In step one, the alkyne-containing nucleoside is fed to cells or animals and actively incorporated into nascent RNA. The small size of the tag enables efficient incorporation of the modified nucleoside into RNA, but not into DNA. Detection utilizes the chemoselective ligation or “click" reaction between and azide and an alkyne where the modified RNA is detected with a corresponding azide-containing dye. With its dimunitive “footprint", the Click-iT® detection molecule can easily penetrate complex samples and leaves open the possibility of multiplex analyses with other probes, including antibodies for the detection of RNA-interactive proteins for deeper biological insights.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
New RNA Synthesis Assay
Color
Green
Format
96-well plate
Emission
Visible
Excitation
Visible
Excitation/Emission
495⁄519
Label Type
Alexa Fluor Dyes
Shipping Condition
Room Temperature
Product Line
Alexa Fluor™, Click-iT™, Molecular Probes™
For Use With (Equipment)
High Content Instrument
Label or Dye
Alexa Fluor™ 488
Green Features
Less hazardous
Detection Method
Fluorescent

Contents & Storage

The kit contains sufficient material for 2 X 96-well plates. Store at 2-6°C, dessicate and protect from light.

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