Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit - FAQs

Yes, we offer the Alexa Fluor 488 azide (Component B) from the Click-iT RNA Alexa Fluor 488 Imaging Kit (Cat. No. C10329) as a standalone product. The product is sold separately, under the product name Alexa Fluor 488 Azide (Alexa Fluor 488 5-Carboxamido-(6-Azidohexanyl), Bis(Triethylammonium Salt)), 5-isomer (Cat. No. A10266).

Please note, the standalone Alexa Fluor 488 Azide comes in a greater quantity than the Click-iT RNA Alexa Fluor 488 Imaging Kit. The concentration of Alexa Fluor azide present in the Click-iT RNA Alexa Fluor 488 Imaging Kit is confidential, so you will need to optimize the concentration of Alexa Fluor Azide for your experiment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Other than the EdU (Component A), DMSO (Component C), and Click-iT EdU buffer additive (Component F or G), all other components in the respective kits are not interchangeable. The types of reagents and amounts provided per kit were optimized either for imaging or flow cytometry. Using the imaging reagents may result in an excessive level of signal for flow cytometry detection and using the flow cytometry reagents may result in sub-optimal signal for imaging.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. The cell must possess a pyrimidine salvage pathway; without this pathway, EU does not become phosphorylated to allow incorporation during RNA synthesis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.