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Click-IT® TAMRA DIBO Alkyne, for copper free click chemistry detection of azide This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: C10410

Molecular Probes™  Related applications: Labeling Chemistry

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Description

The fluorescent TAMRA DIBO alkyne is reactive with azides via a copper-free "click" reaction.

Please consider this copper-free variation to our copper requiring alkynes if you are staining the surface of live cells or have concerns about native protein function loss with copper in cell extracts. Copper can damage fluorescent proteins, Quantum Dot nanocyrstals, certain enzymes and photoproteins like RPE. The DIBO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds. TAMRA (tetramethylrhodamine) labeled proteins can be analyzed by 1D or 2D gel electrophoresis, Western blot with an anti-TAMRA antibody, or by Mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Red
Detection Location: In-Cell Detection, In-Gel Detection
Detection Method: Fluorescent, Gel Electrophoresis
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 542⁄568
For Use With (Equipment): Fluorescent Imager, Gel Scanner
Form: Solid
Green Features: Less hazardous
Label or Dye: TAMRA™
Product Size: 500 µg
Shipping Condition: Dry Ice
Solubility: DMSO (Dimethylsulfoxide)

Contents & storage

Store at ≤ -20°C. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time

Documents

Manuals & protocols