Click-IT® Biotin DIBO Alkyne, for copper free click chemistry This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: C10412

Molecular Probes™  Related applications: Labeling Chemistry

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The biotin DIBO alkyne is reactive with cells or proteins containing any number of azide modified molecules via a copper-free "click" reaction.

Please consider this copper free variation to our copper requiring alkynes if you are staining the surface of live cells or have concerns about native protein function loss with copper in cell extracts. Copper can damage fluorescent proteins, Quantum Dot nanocyrstals, certain enzymes and photoproteins like RPE. Once incorporated the biotin moiety can be detected with streptavidin or more commonly used for isolation with for example Dynal® Streptavidin magnetic beads.

Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur. With the elimination of copper, no detectable damage to cells or proteins. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Solid
Color: Biotin
Solubility: DMSO (Dimethylsulfoxide)
Label or Dye: Unconjugated
Product Size: 1 mg
Detection Method: Fluorescent
Detection Location: In-Gel Detection, In-Cell Detection
For Use With (Equipment): Fluorescence Microscope, High Content Instrument, Flow Cytometer, Confocal Microscope
Green Features: Less hazardous
Shipping Condition: Dry Ice

Contents & storage

Store at ≤ -20°C. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time

Documents

Manuals & protocols