Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number:  C10427

Related applications:

Cell Analysis

| Cell Analysis

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Description

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent® Caspase-3/7 Green Detection Reagent, as well as SYTOX® AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

View a selection guide for all apoptosis assays for flow cytometry

CellEvent® Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ~511/533 nm. When used together with the SYTOX® AADvanced™ Dead Cell Stain, apoptotic cells can be easiliy discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis. For more information, see Apoptosis Reagents in Flow Cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
Excitation/Emission: SYTOX® AADvanced™: 546⁄ 647, CellEvent™ Caspase 3/7 Green Detection Reagent: 511⁄533
Flow Cytometer Laser Lines: 488
Format: Tube(s)
Label Type: Other Label(s) or Dye(s)
Label or Dye: CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX® AADvanced dead cell stain
No. of Reactions: 100
Product Line: CellEvent™
Shipping Condition: Wet Ice
Solubility: DMSO (Dimethylsulfoxide)
For Use With (Equipment): Flow Cytometer

Contents & storage

Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX® AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20 °C, desiccated, and protected from light.