Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number: C10427
Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number: C10427
Catalog Number
C10427
Unit Size
100 assays
Price (USD)
423.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
20 assays
Price (USD)
122.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10427100 assays
423.00
Your Price:
***
C1074020 assays
122.00
Your Price:
***
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The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Fluorescent
Excitation/Emission
SYTOX™ AADvanced™: 546⁄ 647, CellEvent™ Caspase 3/7 Green Detection Reagent: 511⁄533
Flow Cytometer Laser Lines
488
For Use With (Equipment)
Attune™ Acoustic Focusing Cytometer, Flow Cytometer
Format
Tube(s)
Label or Dye
CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX™ AADvanced dead cell stain
Product Line
CellEvent™
Shipping Condition
Wet Ice
Solubility
DMSO (Dimethylsulfoxide)
For Use With (Equipment)
Flow Cytometer
Quantity
100
Format
Kit
Product Type
Caspase 3/7 Green Flow Cytometry Assay Kit

Contents & Storage

Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX™ AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20 °C, desiccated, and protected from light.

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