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CellEvent™ Caspase-3/7 Green Detection Reagent (lyophilized), 1 Vial - FAQs

CellEvent™ Caspase-3/7 Green Detection Reagent (lyophilized), 1 Vial - FAQs

View additional product information for CellEvent™ Caspase-3/7 Detection Reagents - FAQs (C10430, C10723, C10432, C10431, C10434, C10423, C10433)

4 product FAQs found

How long can I measure the Cell Event Caspase-3/7 Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) signal for the kinetic assay?

We have tested Caspase 3/7 Green Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) for up to 48 hours and observed no toxicity or stability issues with the reagent. If the samples are fixed at the end of the time course the signal will be retained for days. For live cells, apoptotic cells will round up and float away before the loss of fluorescence signal from CellEvent.

The following reference reports a visible signal for up to 72 hrs:

Sambi M, Samuel V, Qorri B, Haq S, Burov SV, Markvicheva E, Harless W, Szewczuk MR. A Triple Combination of Metformin, Acetylsalicylic Acid, and Oseltamivir Phosphate Impacts Tumour Spheroid Viability and Upends Chemoresistance in Triple-Negative Breast Cancer. Drug Des Devel Ther. 2020 May 25;14:1995-2019. doi: 10.2147/DDDT.S242514. PMID: 32546966; PMCID: PMC7260544.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use other nucleic acid stains with CellEvent Caspase-3/7 Detection Reagents (Cat. Nos. C10430, C10431, C10432, C10433, C10434)?

We do not recommend using other nucleic acid stains with CellEvent Caspase-3/7 Detection Reagents (Cat. Nos. C10430, C10431, C10432, C10433, C10434). The application of other nucleic acid stains may result in inadequate detection of the caspase-cleaved reagent. Other nucleic acid stains might limit binding of the CellEvent Caspase-3/7 Green or Red Detection Reagent (competition) or spectrally interact with the reagents (quenching, FRET, etc.).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Upon cleavage by caspases, can the CellEvent Caspase-3/7 Detection Reagents (Cat. Nos. C10430, C10431, C10432, C10433, C10434) also stain RNA in the cytoplasm?

It is possible for CellEvent Caspase-3/7 Detection Reagents (Cat. Nos. C10430, C10431, C10432, C10433, C10434) to stain RNA in the cytoplasm upon cleavage by caspases, but the extent of RNA staining should be minimal.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the nucleic acids-binding dyes attached to the DEVD peptide of the CellEvent Caspase-3/7 Detection Reagents (Cat. Nos. C10430, C10431, C10432, C10433, C10434)?

The identity of the nucleic acid dyes attached to the DEVD peptide of the CellEvent Caspase-3/7 Detection Reagents (Cat. Nos. C10430, C10431, C10432, C10433, C10434) are proprietary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.