CellROX™ Orange Reagent, for oxidative stress detection

Citations & References (18)

Invitrogen™

CellROX™ Orange Reagent, for oxidative stress detection

Catalog Number	Quantity
C10443	5 x 50 μL

Catalog number C10443

Price (MXN)

Quantity:

5 x 50 μL

CellROX™ Orange Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells. This cell-permeant dye is non-fluorescent while in a reduced state and exhibits bright orange fluorescence upon oxidation by reactive oxygen species (ROS), with absorption/emission maxima of ∼545/565 nm. While this reagent is not formaldehyde-fixable, it can be multiplexed with other live cell-compatible probes such as GFP, CellEvent™ Caspase 3/7 Green reagent, Image-iT™ DEAD Green, Green and Deep Red MitoTracker™ dyes, and NucBlue™ reagent.

CellROX™ Orange Reagent is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), and flow cytometery. This reagent is also compatible with the Tali™ Image-based Cytometer.

In addition, CellROX™ Orange Reagent is:

Compatible with other live cell probes, including GFP, and may be added directly to serum-containing growth media
Provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
Provided as five single-use vials containing enough total reagent for five 96-well plates or 100 coverslips

Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to scavenge them. ROS play an important role in the progression of several diseases, including inflammation, atherosclerosis, aging, and age-related degenerative disorders.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorOrange

Concentration2.5 mM stabilized solution in DMSO

For Use With (Equipment)Imaging, HCS, Cytometer

FormatFrozen

Quantity5 x 50 μL

Detection MethodLive Cell Imaging

Excitation/Emission545/565 nm

IndicatorOxidative stress

Product LineCellROX

Unit SizeEach

Contents & Storage

Store at ≤–20°C. Protect from light and desiccate.

Frequently asked questions (FAQs)

What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference: http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am trying to label my cells with a reactive oxygen species (ROS) indicator dye, but I am not seeing a significant difference in signal. What could be happening?

First, make sure you have both a negative (untreated) and positive (ROS-induced) sample to compare. A good positive control can be the use of 100 µM menadione for one hour or 50 µM nefazodone for 24 hours. H2O2 can also be used, though it does not work well for CellROX dyes. Some dyes, such as H2DCFDA, require esterase cleavage, so don't incubate in the presence of serum (which contains esterases that can prematurely cleave the dye). If your positive control does not show significant change compared to the negative control, try increasing the concentration and label time for the dye. Our manuals give starting recommendations. Be sure to image your live cells as soon as possible. Only two dyes (CellROX Green and CellROX Deep Red) are retained with formaldehyde fixation. Finally, make sure you are using filters and instrument settings to match the excitation and emission spectra of the dye.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (18)

Citations & References

Abstract

MicroRNA-27 (miR-27) Targets Prohibitin and Impairs Adipocyte Differentiation and Mitochondrial Function in Human Adipose-derived Stem Cells.

Authors:Kang T, Lu W, Xu W, Anderson L, Bacanamwo M, Thompson W, Chen YE, Liu D,

Journal:

PubMed ID:24133204

Prohibitin (PHB) has been reported to play a crucial role in adipocyte differentiation and mitochondrial function. However, the regulative mechanism of PHB during adipogenesis remains unclear. In this study, we determined that the levels of both microRNA (miR)-27a and miR-27b were down-regulated following adipogenic induction of human adipose-derived stem cells, ... More

Mitohormesis in Hypothalamic POMC Neurons Mediates Regular Exercise-Induced High-Turnover Metabolism.

Authors:

Journal:Cell Metab

PubMed ID:33535098

Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages.

Authors:Gasparrini M, Forbes-Hernandez TY, Giampieri F, Afrin S, Alvarez-Suarez JM, Mazzoni L, Mezzetti B, Quiles JL, Battino M

Journal:Food Chem Toxicol

PubMed ID:28130090

'A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects ... More

Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling.

Authors:

Journal:Cell Metab

PubMed ID:29972798

Integrative Omics Analyses Reveal Epigenetic Memory in Diabetic Renal Cells Regulating Genes Associated With Kidney Dysfunction.

Authors:

Journal:Diabetes

PubMed ID:32747424

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