Invitrogen™

CellROX™ Orange Flow Cytometry Assay Kit

Catalog number: C10493
Invitrogen™

CellROX™ Orange Flow Cytometry Assay Kit

Catalog number: C10493
Catalog Number
C10493
Unit Size
100 assays
Price (USD)
371.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10493100 assays
371.00
Your Price:
***
Product Overview
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The CellROX™ Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Orange Reagent, as well as SYTOX™ Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX® Reagents and Kits.

The CellROX™ Orange Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Orange Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 545/565 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Red Dead Cell Stain, oxidatively stressed and nonstressed cells are reliably distinguished from dead cells by flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Flow Cytometer Laser Lines
633/635, 532
Label or Dye
CellROX™ Orange Reagent
No. of Tests
100
Physical Form
Tube(s)
Shipping Condition
Wet Ice
Detection Method
Fluorescent
Excitation/Emission
CellROX™ orange: 545⁄565, SYTOX™ red: 640⁄658
Product Line
CellROX™

Contents & Storage

Contains 1 vial CellROX™ Orange Reagent (25 µL), 1 vial SYTOX™ red dead cell stain (100 µL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water), and 1 vial DMSO (200 µL).

Store kit at -5 to -30°C, protected from light. CellROX™ reagent is air sensitive.

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