Invitrogen™

CellROX™ Orange Flow Cytometry Assay Kit

Catalog number:  C10493

Related applications:

Cell Viability, Proliferation & Function

| Flow Cytometry

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Description

The CellROX™ Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Orange Reagent, as well as SYTOX™ Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX® Reagents and Kits.

The CellROX™ Orange Flow Cytometry Assay Kit features:

• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

The CellROX™ Orange Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 545/565 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Red Dead Cell Stain, oxidatively stressed and nonstressed cells are reliably distinguished from dead cells by flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Tube(s)
Shipping Condition: Wet Ice
Product Line: CellROX®
Detection Method: Fluorescent
Excitation/Emission: CellROX® orange: 545⁄565, SYTOX® red: 640⁄658
Flow Cytometer Laser Lines: 633/635, 532
Label or Dye: CellROX® Orange Reagent
No. of Tests: 100

Contents & storage

Contains 1 vial CellROX® Orange Reagent (25 µL), 1 vial SYTOX® red dead cell stain (100 µL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 µL, 70% solution in water), and 1 vial DMSO (200 µL).

Store kit at -5 to -30°C, protected from light. CellROX® reagent is air sensitive.