Invitrogen™

CellLight™ Actin-GFP, BacMam 2.0

Catalog number: C10506
Invitrogen™

CellLight™ Actin-GFP, BacMam 2.0

Catalog number: C10506
Catalog Number
C10506
Unit Size
100 µL
Price (USD)
129.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 mL
Price (USD)
552.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10506100 µL
129.00
Your Price:
***
C105821 mL
552.00
Your Price:
***
Product Overview
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CellLight™ Actin-GFP, BacMam 2.0, provides an easy way to label actin with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight™ fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses GFP fused to human actin with almost no cytotoxic effects. Study the dynamic action of actin filaments in live cells and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight™ constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight™ Technology is:
Fast and convenient: simply add CellLight™ reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight™ reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight™ Actin-GFP, BacMam 2.0, is a fusion construct of human actin and emGFP, providing accurate and specific targeting to cellular actin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight™ reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Specifications

Color
Green
Excitation Wavelength Range
488⁄510
Dye Type
GFP (EmGFP)
For Use With (Equipment)
Attune™ Acoustic Focusing Cytometer, Confocal Microscope, Floid™ Cell Imaging System, Fluorescence Microscope, Microscope
Quantity
100μL
Detection Method
Fluorescent
Delivery Type
Baculovirus (BacMam System)
Emission
Visible
Form
Liquid
Product Line
CellLight™
Shipping Condition
Wet Ice
Target
Actin
Technique
In Vivo Imaging, Fluorescence Intensity
Label Type
Fluorescent Protein
Product Type
Actin
SubCellular Localization
Actin

Contents & Storage

Store at 2°C to 6°C, protected from light. Do Not Freeze.

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