CellLight™ Tubulin-GFP, BacMam 2.0

CellLight™ Tubulin-GFP, BacMam 2.0 - FAQs

2 product FAQs found

Do CellLight BacMam 2.0 reagents work on all cell types?

BacMam delivery technology works with over 90 mammalian cell types. However, cells with hematopoietic origin do not show effective transduction with CellLight BacMam 2.0 reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I keep getting low transduction efficiency when using CellLight labeling reagents on my neurons. What can I do to improve the efficiency?

Neurons are more difficult to transduce than many other cells. The main way to improve transduction is to label with a higher number of particles per cell. For primary neurons, it can also help to transduce them at the time of plating rather than on established cultures. There can also be a slower onset of expression in neurons and peak expression often occurs on day 2-3 rather than 16 hours after transduction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.