Invitrogen™

CellLight™ Actin-GFP, BacMam 2.0

Catalog number: C10582
Invitrogen™

CellLight™ Actin-GFP, BacMam 2.0

Catalog number: C10582
Catalog Number
C10582
Unit Size
1 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C105821 mL
-
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CellLight Actin-GFP, BacMam 2.0, provides an easy way to label actin with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses GFP fused to human actin with almost no cytotoxic effects. Study the dynamic action of actin filaments in live cells and multiplex with other fluorescent proteins or organic dyes.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight Technology is:
Fast and convenient: simply add CellLight reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight Actin-GFP, BacMam 2.0, is a fusion construct of human actin and emGFP, providing accurate and specific targeting to cellular actin-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Green
Excitation Wavelength Range
488⁄510
Dye Type
GFP (EmGFP)
For Use With (Equipment)
Confocal Microscope, Fluorescence Microscope
Quantity
1 vial
Detection Method
Fluorescent
Emission
Visible
Form
Liquid
Product Line
CellLight™
Shipping Condition
Wet Ice
Target
Actin, Cytoskeleton
Technique
Fluorescence Intensity
Label Type
Fluorescent Dye
Product Type
Actin
SubCellular Localization
Actin, Cytoskeleton

Contents & Storage

Store at 2°C to 6°C, protected from light. Do Not Freeze.

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