CellLight™ Early Endosomes-GFP, BacMam 2.0
Invitrogen™
CellLight™ Early Endosomes-GFP, BacMam 2.0
CellLight Early Endosomes-GFP, BacMam 2.0, provides an easy way to label early endosomes with green fluorescent protein (GFP) in liveRead more
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Catalog number C10586
Price (USD)/ 1 mL
600.65
Online exclusive
626.00 
Save 25.35 (4%)
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Price (USD)/ 1 mL
600.65
Online exclusive
626.00 
Save 25.35 (4%)
Add to cart
CellLight™ Early Endosomes-GFP, BacMam 2.0
Catalog numberC10586
Price (USD)/ 1 mL
600.65
Online exclusive
626.00 
Save 25.35 (4%)
-
Add to cart
CellLight Early Endosomes-GFP, BacMam 2.0, provides an easy way to label early endosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Rab5a. You can observe early endosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight Technology is:
Fast and convenient: simply add CellLight reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight Early Endosomes-GFP, BacMam 2.0, is a fusion construct of Rab5a and emGFP, providing accurate and specific targeting to cellular Early Endosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Excitation Wavelength Range488⁄510
Dye TypeGFP (EmGFP)
For Use With (Equipment)Confocal Microscope, Fluorescence Microscope
Quantity1 mL
Detection MethodFluorescence
EmissionVisible
FormLiquid
Product LineCellLight™
Shipping ConditionWet Ice
TechniqueFluorescence Intensity
Label TypeFluorescent Dye
Product TypeEndosomes Label
SubCellular LocalizationEndosomes
Unit Size1 mL
Contents & Storage
Store at 2°C to 6°C, protected from light. Do Not Freeze.