Invitrogen™

Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection

Green features
Catalog number: C10618
Invitrogen™

Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection

Green features
Catalog number: C10618
Detect more apoptotic cells in tissues and cultured cell samples with the Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection, which offers Alexa Fluor 488, 594, and 647 fluorescent dye options. This in situ apoptosis detection kit is optimized for tissue or cell samples and the dyes can be multiplexed with other dyes or proteins, such as GFP and RFP, and incorporated more readily into complex molecules due to their smaller size (compared with antibodies). This TUNEL assay kit is also very flexible and can be used to test 1–50 samples in a single experiment.
 
Catalog Number
C10618
Unit Size
1 kit
Color
Red
Label or Dye
Alexa Fluor™ 594
No. of Reactions
50 coverslips
Price (USD)
Price: 764.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionClick-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Alexa Fluor™ 594 dye
FormatCoverslip
Product TypeTUNEL Assay
Excitation/Emission590/617
No. of Reactions50 coverslips
ColorRed
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor™ Dyes
Storage RequirementsStore at <20°C and protect from light.
Green FeaturesLess hazardous
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Color
Green
Label or Dye
Alexa Fluor™ 488
No. of Reactions
50 coverslips
Price (USD)
Price: 778.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionClick-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Alexa Fluor™ 488 dye
FormatCoverslip
Product TypeTUNEL Assay
Excitation/Emission490/525
No. of Reactions50 coverslips
ColorGreen
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor™ Dyes
Storage RequirementsStore at <20°C and protect from light.
Green FeaturesLess hazardous
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Color
Far-Red
Label or Dye
Alexa Fluor™ 647
No. of Reactions
50 coverslips
Price (USD)
Price: 768.00
Your Price:
Availability
-
Full specifications
Quantity
DescriptionClick-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Alexa Fluor™ 647 dye
FormatCoverslip
Product TypeTUNEL Assay
Excitation/Emission650/665
No. of Reactions50 coverslips
ColorFar-Red
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor™ Dyes
Storage RequirementsStore at <20°C and protect from light.
Green FeaturesLess hazardous
Unit Size1 kit
Showing 3 of 3
Catalog NumberSpecificationsUnit SizeColorLabel or DyeNo. of ReactionsPrice (USD)AvailabilityQuantity
C10618Full specifications
1 kitRedAlexa Fluor™ 59450 coverslips
Price: 764.00
Your Price:
-
DescriptionClick-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Alexa Fluor™ 594 dye
FormatCoverslip
Product TypeTUNEL Assay
Excitation/Emission590/617
No. of Reactions50 coverslips
ColorRed
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor™ Dyes
Storage RequirementsStore at <20°C and protect from light.
Green FeaturesLess hazardous
Unit Size1 kit
C10617Full specifications
1 kitGreenAlexa Fluor™ 48850 coverslips
Price: 778.00
Your Price:
-
DescriptionClick-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Alexa Fluor™ 488 dye
FormatCoverslip
Product TypeTUNEL Assay
Excitation/Emission490/525
No. of Reactions50 coverslips
ColorGreen
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor™ Dyes
Storage RequirementsStore at <20°C and protect from light.
Green FeaturesLess hazardous
Unit Size1 kit
C10619Full specifications
1 kitFar-RedAlexa Fluor™ 64750 coverslips
Price: 768.00
Your Price:
-
DescriptionClick-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Alexa Fluor™ 647 dye
FormatCoverslip
Product TypeTUNEL Assay
Excitation/Emission650/665
No. of Reactions50 coverslips
ColorFar-Red
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor™ Dyes
Storage RequirementsStore at <20°C and protect from light.
Green FeaturesLess hazardous
Unit Size1 kit
Showing 3 of 3
The Click-iT Plus TUNEL Alexa Fluor 488, 594, and 647 assays for in situ apoptosis detection can detect apoptotic cells in tissue and cultured cell samples through the incorporation of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed “click” reaction using conditions mild enough to preserve the emitted fluorescent signal from GFP or RFP.

Other advantages of the Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection include:
• Optimized for the detection of apoptotic cells in either tissue or cell samples
• Multiplex enabled—optimized to work with fluorescent dyes or proteins such as GFP and RFP
• Improved TUNEL assay—better label incorporation due to small reactive moiety
• Bright apoptotic signal—uses Alexa Fluor dyes, resulting in a stable, non-photobleaching fluorescent signal
• Flexibility—the assay can be configured to test 1–50 samples at a time

Fragmentation of cellular DNA is a hallmark of apoptosis. The TUNEL assay is the most widely used method to detect fragmented DNA in apoptotic cells or tissue samples. The TUNEL assay begins with incorporation of modified dUTP at the 3’-OH end of the fragmented DNA. The dUTP modification is often the addition of a fluorophore. Due to the size of the fluorophore, the modified dUTP can display lower than expected incorporation rates, which can affect the sensitivity of the TUNEL assay. Additionally, many fluorophores used in currently available TUNEL assay kits suffer from photobleaching and fluorescent spectral overlap issues, both of which reduce the sensitivity of and ability to multiplex the assay.

The Click-iT Plus TUNEL assay was developed to address these issues. The assay uses dUTP modified with an alkyne group (a small bio-orthogonal functional group), allowing the nucleotide to be more readily incorporated. After incorporation, a highly specific click reaction between the alkyne group and an Alexa Fluor picolyl azide fluorescent dye, and subsequent detection of that dye, results in a sensitive and specific assay for the detection of apoptotic cells or tissue samples. Because of its gentle reaction conditions, the Click-iT Plus TUNEL assay enables multiplexing with fluorescent proteins or dyes.

The Click-iT Plus TUNEL assay has been validated with several different formalin-fixed paraffin-embedded tissue types. In all cases, its ability to multiplex with fluorescent proteins and dyes was preserved. Additionally, the ability to stain actin using fluorescent-labeled phalloidin was also preserved.

The Click-iT Plus TUNEL assay contains all the reagents needed to detect apoptotic cells from either tissue or cell samples. The reagents supplied in this kit can be used to test 50 samples and can be configured to test 1–50 samples at a time.
For Research Use Only. Not for use in diagnostic procedures.