Invitrogen™

CellEvent™ Senescence Green Flow Cytometry Assay Kit

Catalog number:  C10840

 Related applications:

Flow Cytometry

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The CellEvent Senescence Green Flow Cytometry Assay Kit enables flow cytometric detection of cellular senescence via β-galactosidase hydrolysis. The kit contains an optimized buffer and a fluorescein-based reagent that contains two galactoside moieties, making it a specific target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The enzyme-cleaved product is retained within the cell due to covalent binding of intracellular proteins and emits a fluorogenic signal that has absorption/emission maxima of 490/514 nm.

CellEvent Senescence Green Flow Cytometry Assay Kit features include:
• Fluorescent detection of β-galactosidase hydrolysis by flow cytometry
• Fixed cell assay
• Multi-color compatibility

When normal cells reach the end of their limited replicative lifespan, commonly referred to as the Hayflick limit, they enter into a senescent phase where they remain metabolically active without undergoing cell death processes. These senescent cells adopt a specific phenotypic state that includes the appearance of multi-nucleated cells, increased vacuolization, expression of pH-dependent β-galactosidase, and morphological changes where cells become enlarged and extended. Through a variety of mechanisms, they may play a role in tumor suppression, tumor progression, aging, and tissue repair.

β-galactosidase resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The activity is optimal under lysosomal pH 4, but conventional assays measure at pH 6. It has been shown that normalized β-galactosidase activity is twice as high in senescent cells as in presenescent cells regardless of the pH value used for testing.

Traditionally, the colourimetric substrate for β-galactosidase, 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (x-gal), has long been used to detect metabolic activity in cells in vitro. Upon hydrolysis by β-galactosidase, x-gal converts to a blue precipitate, which is not suitable for flow cytometric assays. Therefore, we have developed a sensitive, fluorescent substrate for β-galactosidase that can be used in flow cytometry assays for the detection of senescent cells.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
For Use With (Equipment): Flow Cytometer
Sub-Cellular Localization: Lysosomes
Detection Method: Fluorescent
Excitation⁄Emission (nm): 488⁄514
Product Line: CellEvent™
Product Size: 50 assays
Shipping Condition: Room Temperature

Contents & storage

Store at 2–8°C and protect from light.

Documents

Manuals & protocols