Invitrogen™

CellEvent™ Senescence Green Flow Cytometry Assay Kit

Catalog number: C10841
Invitrogen™

CellEvent™ Senescence Green Flow Cytometry Assay Kit

Catalog number: C10841
Catalog Number
C10841
Unit Size
200 assays
Price (USD)
490.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
50 assays
Price (USD)
271.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10841200 assays
490.00
Your Price:
***
C1084050 assays
271.00
Your Price:
***
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The CellEvent Senescence Green Flow Cytometry Assay Kit enables flow cytometric detection of cellular senescence via β-galactosidase hydrolysis. The kit contains an optimized buffer and a fluorescein-based reagent that contains two galactoside moieties, making it a specific target for β-galactosidase. Activation of β-galactosidase is commonly used as a biomarker for senescent cells. This hydrolase enzyme resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The enzyme-cleaved product is retained within the cell due to covalent binding of intracellular proteins and emits a fluorogenic signal that has absorption/emission maxima of 490/514 nm.

CellEvent Senescence Green Flow Cytometry Assay Kit features include:
• Fluorescent detection of β-galactosidase hydrolysis by flow cytometry
• Fixed cell assay
• Multi-color compatibility

When normal cells reach the end of their limited replicative lifespan, commonly referred to as the Hayflick limit, they enter into a senescent phase where they remain metabolically active without undergoing cell death processes. These senescent cells adopt a specific phenotypic state that includes the appearance of multi-nucleated cells, increased vacuolization, expression of pH-dependent β-galactosidase, and morphological changes where cells become enlarged and extended. Through a variety of mechanisms, they may play a role in tumor suppression, tumor progression, aging, and tissue repair.

β-galactosidase resides in lysosomes and converts β-galactosides into monosaccharides under acidic pH conditions. The activity is optimal under lysosomal pH 4, but conventional assays measure at pH 6. It has been shown that normalized β-galactosidase activity is twice as high in senescent cells as in presenescent cells regardless of the pH value used for testing.

Traditionally, the colorimetric substrate for β-galactosidase, 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (x-gal), has long been used to detect metabolic activity in cells in vitro. Upon hydrolysis by β-galactosidase, x-gal converts to a blue precipitate, which is not suitable for flow cytometric assays. Therefore, we have developed a sensitive, fluorescent substrate for β-galactosidase that can be used in flow cytometry assays for the detection of senescent cells.

Specifications

Color
Green
Detection Method
Fluorescent
For Use With (Application)
Flow Cytometry
For Use With (Equipment)
Flow Cytometer
Product Type
Flow Cytometry Assay Kit
Emission
488/514
Product Line
CellEvent™
Quantity
200 Assays
Shipping Condition
Room Temperature

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