Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases. This dye is also available as 1 mg of the solid (C-1430) and resuspended in DMSO (C-3099). For a longer wavelength version of this dye, check out our new CellTrace calcein red-orange AM (C-34851).
For Research Use Only. Not for use in diagnostic procedures.
Other Label(s) or Dye(s)
Cell Tracker Compounds, Cell Labeling Reagents
Contents & Storage
Store in freezer -5°C to -30°C.
Rat lung cells. LIVE/DEAD® Viability/Cytotoxicity Kit, ethidium homodimer-1 and calcein AM.
Jurkat cells incubated with either green-fluorescent calcein AM, CellTrace™ calcein red-orange AM or calcein blue AM.
Loading and retention characteristics of intracellular marker dyes.
BPAE cells stained with Image-iT® LIVE Mitochondrial Transition Pore Assay Kit.