5(6)-CFDA (5-(and-6)-Carboxyfluorescein Diacetate), mixed isomers

5(6)-CFDA es un sustrato de esterasa que penetra en las células y que puede servir como sonda de viabilidad queMás información

Número de catálogo	Cantidad
C195	100 mg

Número de catálogo C195

Precio (MXN)

Cantidad:

100 mg

5(6)-CFDA es un sustrato de esterasa que penetra en las células y que puede servir como sonda de viabilidad que mide tanto la actividad enzimática, que es necesaria para activar su fluorescencia, como la integridad célula-membrana, que es necesaria para la retención intracelular de su producto fluorescente. Tras la hidrólisis por esterasas intracelulares, este éster AM también produce carboxifluoresceína. En comparación con la fluoresceína, la carboxifluoresceína contiene cargas negativas adicionales y, por lo tanto, se conserva mejor en las células.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de coloranteOtras etiquetas o colorantes

Cantidad100 mg

Tipo de reactivoCFDA y compuestos relacionados

Condiciones de envíoTemperatura ambiente

Tipo de producto5(6)-CFDA

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador entre -5 °C y -30 °C

Citations & References (185)

Citations & References

Abstract

Bacteria in post-glacial freshwater sediments.

Authors:Miskin I, Rhodes G, Lawlor K, Saunders JR, Pickup RW

Journal:Microbiology

PubMed ID:9782490

Prokaryote communities in post-glacial profundal freshwater sediments of Windermere, representing 10-12,000 years of deposition, were examined for culturability, viability and community structure. The potential for active geochemical cycles was inferred from the presence of specific groups of bacteria. Direct count procedures revealed 10(12) cells (g dry wt sediment)-1 in the ... More

5-Carboxyfluorescein diacetate as a probe for measuring the growth of keratinocytes.

Authors:Hanthamrongwit M, Reid WH, Courtney JM, Grant MH

Journal:Hum Exp Toxicol

PubMed ID:8086226

There is a requirement for a convenient and reliable method for evaluating the growth rate of human keratinocytes cultured on collagen-based substrates. Therefore, three methods of determining cell growth were first used to quantify the growth rate of the well-characterised L929 mouse fibroblast cell line on tissue culture plastic and ... More

Fluorescent indicators of ion concentrations.

Authors:Tsien RY

Journal:Methods Cell Biol

PubMed ID:2538708

Rapid assessment of physiological status in Escherichia coli using fluorescent probes.

Authors:Porter J, Edwards C, Pickup RW

Journal:J Appl Bacteriol

PubMed ID:7592133

'Rapid and direct viability assessment of Escherichia coli in filtered, sterile lake water was possible using multiparameter flow cytometry. Fluorescent dyes were used as probes for different cellular functions (membrane potential, membrane integrity and intracellular enzyme activity), which were correlated with the ability of the cells to respond to nutrient ... More

Intercellular adhesion mediated by human muscle neural cell adhesion molecule: effects of alternative exon use.

Authors:Pizzey JA, Rowett LH, Barton CH, Dickson G, Walsh FS

Journal:J Cell Biol

PubMed ID:2532218

'Mouse 3T3 fibroblasts were permanently transfected with cDNAs encoding isoforms of the neural cell adhesion molecule (N-CAM) present in human skeletal muscle and brain. Parental and transfected cells were then used in a range of adhesion assays. In the absence of external shear forces, transfection with cDNAs encoding either transmembrane ... More

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