Invitrogen™

SiteClick™ sDIBO Alkyne Kits for Antibody Labeling

Catalog number: C20029
Invitrogen™

SiteClick™ sDIBO Alkyne Kits for Antibody Labeling

Catalog number: C20029
Generate site-specific labeled antibody conjugates with SiteClick sDIBO Alkynes for Antibody Labeling. Our SiteClick sDIBO Antibody Labeling kits are optimized to be used in conjunction with the SiteClick Azido Modification kits (S20026, S10900, or S10901 sold separately) and contain sufficient reagents to perform a conjugation reaction of a SiteClick sDIBO Alkyne label using 100 µg, 1 mg, or 5 mg of azido-modified IgG antibody. The SiteClick sDIBO Alkynes for Antibody Labeling employs copper-free click chemistry to covalently link a dibenzocyclooctyne (sDIBO) labeled Alexa Fluor dye, pHrodo dye, or biotin to an azido modified antibody. The label, when attached to the antibody, is located far away from its antigen binding domain.
 
Catalog Number
C20029
Unit Size
1 each
Label or Dye
Alexa Fluor 647
Kit Contents
Contains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Labeling Scale
100-250 µg
Price (USD)
261.00
Your Price:
Availability
***
Full specifications
Quantity
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
ColorFar-Red
Emission665
Excitation650
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 647
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeCy5
Unit Size1 each
Catalog Number
Unit Size
1 each
Label or Dye
Alexa Fluor 488
Kit Contents
Contains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Labeling Scale
100-250 µg
Price (USD)
261.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
ColorGreen
Emission525
Excitation490
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 488
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeGFP
Unit Size1 each
Catalog Number
Unit Size
1 each
Label or Dye
primary amine
Kit Contents
Contains 1 vial of SiteClick Amine sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Labeling Scale
100-250 µg
Price (USD)
165.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Labeling TargetAzido modified antibodies
Label or Dyeprimary amine
Product LineSiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Amine sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size1 each
Catalog Number
Unit Size
1 kit
Label or Dye
pHrodo iFL Red
Kit Contents
Contains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Labeling Scale
100-250 µg
Price (USD)
293.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
ColorRed
Emission585
Excitation560
Labeling TargetAzido modified antibodies
Label or DyepHrodo iFL Red
Product LinepHrodo, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeRFP, Texas Red
Unit Size1 kit
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
Biotin
Kit Contents
Contains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Labeling Scale
1 mg
Price (USD)
294.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Labeling TargetAzido modified antibodies
Label or DyeBiotin
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
pHrodo iFL Red
Kit Contents
Contains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Labeling Scale
1 mg
Price (USD)
499.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
ColorRed
Emission585
Excitation560
Labeling TargetAzido modified antibodies
Label or DyepHrodo iFL Red
Product LinepHrodo, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeRFP, Texas Red
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
Alexa Fluor 488
Kit Contents
Contains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Labeling Scale
1 mg
Price (USD)
408.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
ColorGreen
Emission525
Excitation490
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 488
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeGFP
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 1 mg of Ab
Label or Dye
Alexa Fluor 647
Kit Contents
Contains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Labeling Scale
1 mg
Price (USD)
416.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
ColorFar-Red
Emission665
Excitation650
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 647
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeCy5
Unit Sizefor 1 mg of Ab
Catalog Number
Unit Size
for 5 mg of Ab
Label or Dye
Biotin
Kit Contents
Contains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 250 µL DMSO and 1 antibody concentrator
Labeling Scale
5 mg
Price (USD)
624.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale5 mg
Shipping ConditionWet Ice
Labeling TargetAzido modified antibodies
Label or DyeBiotin
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 250 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Sizefor 5 mg of Ab
Catalog Number
Unit Size
for 5 mg of Ab
Label or Dye
pHrodo iFL Red
Kit Contents
Contains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Labeling Scale
5 mg
Price (USD)
962.00
Your Price:
Availability
***
Full specifications
Quantity
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale5 mg
Shipping ConditionWet Ice
ColorRed
Emission585
Excitation560
Labeling TargetAzido modified antibodies
Label or DyepHrodo iFL Red
Product LinepHrodo, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeRFP, Texas Red
Unit Sizefor 5 mg of Ab
Showing 10 of 16Show more
Catalog NumberSpecificationsUnit SizeLabel or DyeKit ContentsLabeling ScalePrice (USD)AvailabilityQuantity
C20029Full specifications
1 eachAlexa Fluor 647Contains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator100-250 µg
261.00
Your Price:
***
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
ColorFar-Red
Emission665
Excitation650
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 647
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeCy5
Unit Size1 each
C20027Full specifications
1 eachAlexa Fluor 488Contains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator100-250 µg
261.00
Your Price:
***
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
ColorGreen
Emission525
Excitation490
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 488
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeGFP
Unit Size1 each
C20031Full specifications
1 eachprimary amineContains 1 vial of SiteClick Amine sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator100-250 µg
165.00
Your Price:
***
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
Labeling TargetAzido modified antibodies
Label or Dyeprimary amine
Product LineSiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Amine sDIBO Alkyne for Antibody Labeling in 25 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Size1 each
C20034Full specifications
1 kitpHrodo iFL RedContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator100-250 µg
293.00
Your Price:
***
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale100-250 µg
Shipping ConditionWet Ice
ColorRed
Emission585
Excitation560
Labeling TargetAzido modified antibodies
Label or DyepHrodo iFL Red
Product LinepHrodo, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeRFP, Texas Red
Unit Size1 kit
S10902Full specifications
for 1 mg of AbBiotinContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator1 mg
294.00
Your Price:
***
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
Labeling TargetAzido modified antibodies
Label or DyeBiotin
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Sizefor 1 mg of Ab
S10903Full specifications
for 1 mg of AbpHrodo iFL RedContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator1 mg
499.00
Your Price:
***
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
ColorRed
Emission585
Excitation560
Labeling TargetAzido modified antibodies
Label or DyepHrodo iFL Red
Product LinepHrodo, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeRFP, Texas Red
Unit Sizefor 1 mg of Ab
S10904Full specifications
for 1 mg of AbAlexa Fluor 488Contains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator1 mg
408.00
Your Price:
***
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
ColorGreen
Emission525
Excitation490
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 488
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 488 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeGFP
Unit Sizefor 1 mg of Ab
S10906Full specifications
for 1 mg of AbAlexa Fluor 647Contains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator1 mg
416.00
Your Price:
***
Chemical ReactivityAzide
Flow Cytometer Laser LinesRed
Labeling MethodClick Chemistry
Labeling Scale1 mg
Shipping ConditionWet Ice
ColorFar-Red
Emission665
Excitation650
Labeling TargetAzido modified antibodies
Label or DyeAlexa Fluor 647
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Alexa Fluor 647 sDIBO Alkyne for Antibody Labeling in 50 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeCy5
Unit Sizefor 1 mg of Ab
S10907Full specifications
for 5 mg of AbBiotinContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 250 µL DMSO and 1 antibody concentrator5 mg
624.00
Your Price:
***
Chemical ReactivityAzide
Labeling MethodClick Chemistry
Labeling Scale5 mg
Shipping ConditionWet Ice
Labeling TargetAzido modified antibodies
Label or DyeBiotin
Product LineAlexa Fluor, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 250 µL DMSO and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Unit Sizefor 5 mg of Ab
S10908Full specifications
for 5 mg of AbpHrodo iFL RedContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator5 mg
962.00
Your Price:
***
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale5 mg
Shipping ConditionWet Ice
ColorRed
Emission585
Excitation560
Labeling TargetAzido modified antibodies
Label or DyepHrodo iFL Red
Product LinepHrodo, SiteClick
Reactive MoietysDIBO Alkyne
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Storage RequirementsStore in refrigerator (2–8°C) and protect from light.
Filter TypeRFP, Texas Red
Unit Sizefor 5 mg of Ab
Showing 10 of 16Show more
Create your own covalently labeled antibody conjugates using the SiteClick sDIBO Alkyne labels, which are intended for use with azido-modified antibodies. SiteClick sDIBO Alkyne Antibody Labeling Kits include the sDIBO Alkyne and the antibody concentrator, enabling copper-free covalent attachment of Alexa Fluor and pHrodo dyes, biotin, or molecules of your choice. When used with the SiteClick Antibody Azido Modification Kit (available separately as Cat. No. S20026, S10900, S10901), labeling occurs on the heavy chain N-linked glycans, far away from the antigen-binding domain, enabling excellent reproducibility between separate labeling reactions and multiple antibody species and subtypes. The SiteClick sDIBO alkyne kits for antibody labeling are optimized for conjugation reactions, covalently linking 100 µg, 1 mg, or 5 mg of azide-modified IgG antibody.

The SiteClick Antibody Azido Modification Kit (Cat. No. S20026, S10900, S10901) is recommended for the attachment of an azide moiety to the heavy chains of an unlabeled IgG antibody. Other engineered antibodies containing azido moieties are also suitable.

When used in conjunction with the SiteClick Antibody Azido Modification Kit, the SiteClick sDIBO alkyne kits for antibody labeling give you the option of trying different fluorescent labels for your antibody, attaching other molecules via streptavidin, or attaching your own molecule via amine-reactive moieties, depending on your assay. Our sDIBO alkynes are improved versions of DIBO cyclooctynes, yielding conjugates that are less 'sticky' and emit lower signal background in biological samples.
For Research Use Only. Not for use in diagnostic procedures.

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Frequently asked questions (FAQs)

Citations & References