Invitrogen™

CyQUANT™ LDH Cytotoxicity Assay

Catalog number: C20300
Invitrogen™

CyQUANT™ LDH Cytotoxicity Assay

Catalog number: C20300
Catalog Number
C20300
Unit Size
200 assays
Price (USD)
160.00
Your Price:
Quantity
Catalog Number
Unit Size
1000 assays
Price (USD)
441.00
Your Price:
Quantity
Catalog NumberUnit SizePrice (USD)Quantity
C20300200 assays
160.00
Your Price:
C203011000 assays
441.00
Your Price:
Product Overview
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The CyQUANT LDH Cytotoxicity Assay is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity. Lactate dehydrogenase (LDH) is a cytosolic enzyme present in many different cell types that is released into the cell culture medium upon damage to the plasma membrane. The CyQUANT LDH Cytotoxicity Assay provides the reagents to accurately and quantitatively measure this extracellular LDH.

Note: The CyQUANT LDH Cytotoxicity Assay (Cat. Nos. C20300 and C20301) are direct replacements for the Pierce LDH Cytotoxicity Assay Kit (Cat. Nos. 88953 and 88954).

CyQUANT LDH Cytotoxicity Assay features include:
Convenient—add-mix-read assay format for adherent and suspension cells, including 3D cell models
Accurate—provides a quantitative measurement of LDH release
Flexible—ideal for high-throughput screening, monitor cytotoxicity from the same sample over time
Robust—uses stable LDH enzyme activity as a cytotoxic marker

LDH is a cytosolic enzyme present in many different cell types and is a well-established and reliable indicator of cellular toxicity. Damage of the plasma membrane results in a release of LDH into the surrounding cell culture medium. This extracellular LDH can be quantified by a coupled enzymatic reaction in which LDH catalyzes the conversion of lactate to pyruvate via NAD+ reduction to NADH. Diaphorase then uses NADH to reduce a tetrazolium salt (INT) to a red formazan product that can be measured at 490 nm. The level of formazan formation is directly proportional to the amount of LDH released into the medium.

The CyQUANT LDH Cytotoxicity Assay provides the reagents needed for the simple, reliable colorimetric quantification of cellular cytotoxicity. The kit can be used with different cell types, including 3D cell models, to measure cytotoxicity mediated by chemical compounds as well as cell-mediated cytotoxicity. Since LDH in the medium is the indicator of cellular cytotoxicity, the assay can be used to monitor cytotoxicity from the same sample over time. To perform the assay, an aliquot of the cell culture medium is transferred to a new plate and the reaction mixture is added. After a 30-minute incubation, the reaction is stopped by adding Stop Solution and absorbance is measured using a microplate reader.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Colorimetric
Format
96-well plate
Product Line
CyQUANT™
Quantity
200 assays
Shipping Condition
Dry Ice
For Use With (Equipment)
Microplate Reader

Contents & Storage

  • Substrate Mixture, 1 vial lyophilizate
  • Assay Buffer, 1 x 600 μL
  • Lysis Buffer, 2.5 mL
  • Stop Solution, 12 mL
  • LDH Positive Control, 6 μL
  • Store at -5 to -30°C, protect from light

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