Sondas fluorescentes CellTracker™
Sondas fluorescentes CellTracker™
Citas y referencias (17)
Invitrogen™

Sondas fluorescentes CellTracker™

Es un colorante fluorescente adecuado para supervisar el movimiento de las células o su ubicación.
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Número de catálogoCantidadTipo de colorante
C21025 mgColorantes BODIPY
C21105 mgCellTracker™ Blue CMAC
C21115 mgCellTracker™ Blue CMHC
C128815 mgCellTracker Blue CMF2HC
C3456520 × 15 μgCellTracker™ Deep Red
C702520 x 50 μgCellTracker Green CMFDA
C29251mgCellTracker Green CMFDA
C3455120 × 50 μgCellTracker Orange CMRA
C29271 mgCellTracker Orange CMTMR
C3455220 × 50 μgCellTracker™ Red CMTPX
C100945 x 0,1 mgThiolTracker™ Violet
Número de catálogo C2102
Precio (MXN)
-
Cantidad:
5 mg
Tipo de colorante:
Colorantes BODIPY
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

  • Este colorante se conserva bien, lo que permite el seguimiento multigeneracional de movimientos celulares.
  • Los espectros de excitación/emisión verde (492/517 nm maxima) son ideales para el multiplexing con proteínas y colorantes rojo fluorescente.
  • Fácil de usar: retire el medio, añada el colorante, incube 30 minutos y obtenga la imagen de las células.
  • Retención de la señal fluorescente superior a >72 horas (normalmente de tres a seis generaciones).
  • Baja citotoxicidad: no afecta a la viabilidad ni a la proliferación
  • El tinte fluorescente de CMAC CellTracker Blue se ha diseñado para atravesar libremente las membranas celulares hasta las células donde se transforma en productos de reacción impermeabilizantes de membranas celulares.
  • El colorante se transfiere a las células hijas, pero no a las celdas adyacentes de una población.
  • Estable, no tóxico a las concentraciones de trabajo, bien conservado en las células, y brillante fluorescente a pH fisiológico

Adhesión celular, análisis celular, proliferación celular, marcado y seguimiento celular, viabilidad celular y citotoxicidad, viabilidad celular, proliferación y función, Imágenes celulares, ensayos de toxicología celular, quimiotaxis y migración celular, descubrimiento y desarrollo de fármacos, marcado celular general, detección de glutatión, detección de alto contenido (HCS), inmunofluorescencia (IF), tinción y detección de inmunofluorescencia, señalización y homeostomía, señalización, identificación y señalización Seguimiento microbiano, estrés nitrooxidativo, ensayos ADME/Tox basados en dianas, detección de pH

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorVerde
DescripciónCellTracker™ Green BODIPY™ Dye, 5 mg
Tipo de coloranteColorantes BODIPY
Emisión529 nm
Intervalo de longitud de onda de excitación522 nm
FormularioDry Powder
Línea de productosBODIPY, CellTracker
Cantidad5 mg
Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaOtras etiquetas o colorantes
Tipo de productoTinte
SubCellular LocalizationCytoplasm
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

Can I use the CellTracker Green BODIPY Dye (Cat. No. C2102) to label proteins?

Although the chloromethyl moiety on the CellTracker Green BODIPY Dye will react with accessible thiols on proteins, it is less reactive than a BODIPY maleimide (e.g., Cat. No. B10250) or BODIPY iodoacetamide (e.g., Cat. No. D6003). For labeling thiols on proteins/peptides, we recommend using either a maleimide or iodoacetamide derivative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Sondas fluorescentes CellTracker™ FAQs

Citations & References (17)

Citations & References
Abstract
Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model.
Authors:Ueda A, Koga M, Ikeda M, Kudo S, Tanishita K
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:15130887
'Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this ... More
Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide.
Authors:Pappolla MA, Sos M, Omar RA, Bick RJ, Hickson-Bick DL, Reiter RJ, Efthimiopoulos S, Robakis NK
Journal:J Neurosci
PubMed ID:9030627
'Studies from several laboratories have generated evidence suggesting that oxidative stress is involved in the pathogenesis of Alzheimer''s disease (AD). The finding that the amyloid beta protein (Abeta) has neurotoxic properties and that such effects are, in part, mediated by free radicals has provided insights into mechanisms of cell death ... More
Fluorescent labelling of intracellular bacteria in living host cells.
Authors:Boleti H, Ojcius DM, Dautry-Varsat A
Journal:J Microbiol Methods
PubMed ID:10802143
The fluorescent reagent, CellTracker, labels metabolically-active cells and was used here to label Chlamydia in vivo during their exponential phase of growth in infected cells. HeLa cells infected with C. psittaci were labelled with the CellTracker reagents between 15 and 48 h post-infection. The fluorescent label accumulated in the host-cell ... More
Transcription factor KLF2 regulates the migration of naive T cells by restricting chemokine receptor expression patterns.
Authors:Sebzda E, Zou Z, Lee JS, Wang T, Kahn ML,
Journal:Nat Immunol
PubMed ID:18246069
The migration patterns of naive and activated T cells are associated with the expression of distinct sets of chemokine receptors, but the molecular basis for this regulation is unknown. Here we identify Krupple-like factor 2 (KLF2) as a key transcriptional factor needed to prevent naive T cells from expressing inflammatory ... More
GAS6 mediates adhesion of cells expressing the receptor tyrosine kinase Axl.
Authors:McCloskey P, Fridell YW, Attar E, Villa J, Jin Y, Varnum B, Liu ET
Journal:J Biol Chem
PubMed ID:9287338
Axl is a receptor tyrosine kinase that contains both immunoglobulin and fibronectin III repeats in its extracellular domain reminiscent of cell adhesion molecules. Expression of the receptor tyrosine kinase Axl in the 32D myeloid cell line permits aggregation of cells in response to treatment with the native ligand GAS6; this ... More
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