Sondas fluorescentes CellTracker™
Sondas fluorescentes CellTracker™
Citas y referencias (43)
Invitrogen™

Sondas fluorescentes CellTracker™

Es un colorante fluorescente adecuado para supervisar el movimiento de las células o su ubicación.
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Número de catálogoCantidadTipo de colorante
C21105 mgCellTracker™ Blue CMAC
C21115 mgCellTracker™ Blue CMHC
C128815 mgCellTracker Blue CMF2HC
C3456520 × 15 μgCellTracker™ Deep Red
C702520 x 50 μgCellTracker Green CMFDA
C29251mgCellTracker Green CMFDA
C21025 mgColorantes BODIPY
C3455120 × 50 μgCellTracker Orange CMRA
C29271 mgCellTracker Orange CMTMR
C3455220 × 50 μgCellTracker™ Red CMTPX
C100945 x 0,1 mgThiolTracker™ Violet
Número de catálogo C2110
Precio (MXN)
-
Cantidad:
5 mg
Tipo de colorante:
CellTracker™ Blue CMAC
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

  • Este colorante se conserva bien, lo que permite el seguimiento multigeneracional de movimientos celulares.
  • Los espectros de excitación/emisión verde (492/517 nm maxima) son ideales para el multiplexing con proteínas y colorantes rojo fluorescente.
  • Fácil de usar: retire el medio, añada el colorante, incube 30 minutos y obtenga la imagen de las células.
  • Retención de la señal fluorescente superior a >72 horas (normalmente de tres a seis generaciones).
  • Baja citotoxicidad: no afecta a la viabilidad ni a la proliferación
  • El tinte fluorescente de CMAC CellTracker Blue se ha diseñado para atravesar libremente las membranas celulares hasta las células donde se transforma en productos de reacción impermeabilizantes de membranas celulares.
  • El colorante se transfiere a las células hijas, pero no a las celdas adyacentes de una población.
  • Estable, no tóxico a las concentraciones de trabajo, bien conservado en las células, y brillante fluorescente a pH fisiológico

Adhesión celular, análisis celular, proliferación celular, marcado y seguimiento celular, viabilidad celular y citotoxicidad, viabilidad celular, proliferación y función, Imágenes celulares, ensayos de toxicología celular, quimiotaxis y migración celular, descubrimiento y desarrollo de fármacos, marcado celular general, detección de glutatión, detección de alto contenido (HCS), inmunofluorescencia (IF), tinción y detección de inmunofluorescencia, señalización y homeostomía, señalización, identificación y señalización Seguimiento microbiano, estrés nitrooxidativo, ensayos ADME/Tox basados en dianas, detección de pH

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorAzul
DescripciónCellTracker™ Blue CMAC Dye, 5 mg
Tipo de coloranteCellTracker™ Blue CMAC
Emisión353 nm
Intervalo de longitud de onda de excitación353⁄466
FormularioDry Powder
Peso molecular209.63
Línea de productosCellTracker
Cantidad5 mg
Tipo de reactivoCompuestos de seguimiento celular, reactivos de etiquetado celular
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaOtras etiquetas o colorantes
Tipo de productoTinte
SubCellular LocalizationCytoplasm
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a total cell stain, similar to HCS CellMask Blue stain, to label cytoplasm and nuclei in live cells. What do you recommend?

You can use a combination of two dyes with overlapping blue emission. For cytoplasm, you can label the cell with CellTracker Blue CMAC. This can be combined with Hoechst 33342 for nuclei. Both dyes are imaged using a standard DAPI filter set.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Sondas fluorescentes CellTracker™ FAQs

Citations & References (43)

Citations & References
Abstract
Profiling antibody responses by multiparametric analysis of primary B cells.
Authors:Story CM, Papa E, Hu CC, Ronan JL, Herlihy K, Ploegh HL, Love JC,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19004776
'Determining the efficacy of a vaccine generally relies on measuring neutralizing antibodies in sera. This measure cannot elucidate the mechanisms responsible for the development of immunological memory at the cellular level, however. Quantitative profiles that detail the cellular origin, extent, and diversity of the humoral (antibody-based) immune response would improve ... More
Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells.
Authors:Yilmaz O, Verbeke P, Lamont RJ, Ojcius DM
Journal:Infect Immun
PubMed ID:16369027
'Porphyromonas gingivalis, an important periodontal pathogen, is an effective colonizer of oral tissues. The organism successfully invades, multiplies in, and survives for extended periods in primary gingival epithelial cells (GECs). It is unknown whether P. gingivalis resides in the cytoplasm of infected cells throughout the infection or can spread to ... More
T cell receptor engagement leads to the recruitment of IBP, a novel guanine nucleotide exchange factor, to the immunological synapse.
Authors:Gupta S, Fanzo JC, Hu C, Cox D, Jang SY, Lee AE, Greenberg S, Pernis AB
Journal:J Biol Chem
PubMed ID:12923183
'Reorganization of the actin cytoskeleton is crucial to the formation and function of the immunological synapse. Rho GTPases are critical mediators of cytoskeletal reorganization, and their activity at the synapse is likely to be stringently regulated. Guanine nucleotide exchange factors (GEFs) belonging to the Dbl family of proteins represent one ... More
Automated image analysis of live/dead staining of the fungus Aureobasidium pullulans on microscope slides and leaf surfaces.
Authors:Nelson CD, Spear RN, Andrews JH
Journal:Biotechniques
PubMed ID:11056819
'An image analysis program and protocol for the identification and enumeration of live versus dead cells of the yeast-like fungus Aureobasidium pullulans was developed for both populations on microscope slides and leaf surfaces. Live cells took up CellTracker Blue, while nonviable cells stained with DEAD Red. Image analysis macro programs ... More
CX3CR1-fractalkine expression regulates cellular mechanisms involved in adhesion, migration, and survival of human prostate cancer cells.
Authors:Shulby SA, Dolloff NG, Stearns ME, Meucci O, Fatatis A
Journal:Cancer Res
PubMed ID:15256432
'Chemokines and their receptors might be involved in the selection of specific organs by metastatic cancer cells. For instance, the CXCR4-SDF-1alpha pair regulates adhesion and migration of breast as well as prostate cancer cells to metastatic sites. In this study, we present the first evidence for the expression of CX3CR1--the ... More
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